E2B (NC_011203) Virus Tagged ORF Clone

CAT#: VC100333

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase [Human adenovirus B], codon optimized for human cell expression, YP_002213831

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100333 represents NCBI reference of YP_002213831 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTGGTCCAAGCACATGGGGCTGGGAGTTTCCACCCTGAAGCAAGTGACCCCGGCTGCCAGCCAC
  CCCGGAGGCGGGCGTGTGAATCTTCTCAGGGTGCCGTCCCTGAACCCACCAGAGCAAGGAGAAGACGCAC
  CACCGCCAGCCATGCAGCTGGGTCACGCGCCGCCCCTGCCGCAAGATGTGCACCGTCAACACCACTGCTT
  AATATGCAGGAGGCCACAGAACCTCCCCCTAGCAAAATGAAGAATAAGGGCACTGTGGTGGCTCCCAAAG
  GCCACGGCACCCTCCAGGCGATTGACATTAGCACCAACGGCCCTGTGGAGATCAAATATTACCTTAACCT
  GCCACACGCCCTGCAGAAGATTATGCAGGTAAACCTTCTCACTCTCCCCACGAACCTGACCCCTCAGCGA
  CTCAGGACCCTCGACAGTTCAGGGCTGAGGGCTCTGGTGTTGGAACTCCGGCCCTGCAGAGCCGAGGTCT
  GGACCTGCCTGCCAAGGGGCCTGGTCTCCATGACAACGATCGAAACCGAAGACGGACACGCCGACGCAGA
  CAATATCGTGAAACGAGAGGTGCAGGCACCCGGCCTGAATTTTCCCCTGAAATTTTTGGTCAAAGGATCC
  CAAGTGCAGCTCATCCACAAAGTGCACCCCGTGAACCGATGTGAATACTGCGGCCGGCTCTACAAGCATA
  AGCACGAATGCAGTGCCAGACGGCGCGAGTTTTATTTCCATCACATAAATAGTCACTCCTCTAATTGGTG
  GCAGGAGATCCAGTTCTTCCCAATTGGATCACACCCGCGGACAGAGCGACTCTTTCTGACCTACGATGTC
  GAGACCTACACCTGGATGGGAAGTTTCGGCAAGCAGCTCATCCCCTTCATGCTGGTGATGAAACTCTCTG
  GGGACCAGCGACTGGTGAACATCGCCTACGACCTTGCAATCAAGTTGAAGTGGGACCGGTGGCGGCAGGA
  CCCCCAGACCTTCTACTGTGTGACACCCGAAAAGATGGCCGTGGGTCAGCATTTCCGCCAGTACCGGGAC
  CAACTGCAGACCGCACTCGCCGTGGACCTGTGGTCATCCTTCCTGAAGGCAAACCCTCACGTACACGAAT
  GGGCGCTGGAGCATTATGCTCTGACCGATCCAACTGACCTCACTTTTGAAGAGCTCAAGAAGCTTCCGCA
  CGTGAGAGGCACTCCTCGGTTCCTGGAGCTTTACATTGTGGGACATAACATAAATGGGTTTGACGAGATT
  GTGCTCGCTGCACAGGTTATTAACAACCGCGCCGAGGTGCCTCAGCCTTTTAAGATCACCCGGAACTTCA
  TGCCAAGAGCTGGAAAGATTCTGTTTAATGATGTCACATTCGCGCTCCCAAACCCTGCCTACAAAAAGCG
  GGTGGATTTCCAGCTTTGGGAGCAGGGCGCTTGTGATGACATAGATTTCAAGTACCAGTTTCTGAAAGTC
  ATGGTTAGAGATACTTTCGCTCTCACCCACACTAGCTTGCGCAATGCTGCACAAGCTTACAGCCTTCCAG
  TTGAAAAAGGATGCTGCCCATATAAGGCCGTAAATCAGTTTTACATGTTGGGAAGCTACCGCGCCGAGAA
  GGATGGCTTCCCGCTCGAGGAATACTGGAAAGATCATGAGGAGTACTTGCTGAACCGGGAGTTGTGGGAG
  AAAAAATCACAACCTAGATACGACATCATCCAGGAAACACTGGACTATTGCGCCCTGGACGTGCTTATCA
  CCGCAGAGTTGGTCGCTAAGCTCCAAGAAAGCTACGCACACTTCATCAGGGACTCTGTCGGCCTGCCACA
  CGCGCATTTCAACATTTTCCAGAGACCTACGATCAGCTCCAACTCCCACGCTATCTTTAGGCAGATCGTG
  TATCGCGCCGAGAAGCCCAACCGAACCAATCTTGGACCAGGGCTGCTTGCTCCCTCTCATGAGCTTTATG
  ATTATGTGAGAGCCAGTATCCGGGGCGGACGGTGCTATCCAACCTATATTGGAATTCTGGAGGAACCTCT
  CTACGTCTACGATATTTGTGGCATGTACGCTTCAGCACTGACGCACCCTATGCCCTGGGGCACCCCTCTC
  AATCCGTACGAGCGCGCTCTGGCAGTCCGGGAGTGGCAGATGACCTTGGATGACCCAGCCACCATTAGTT
  ATTTTGATAAAGATCTGCTGCCAGGCATATTTACAATTGATGCGGATCCCCCTGACGAGTTCATGTTGGA
  CCCCCTTCCTCCATTCTGTTCTCGCAAAGGCGGACGCCTCTGCTGGACCAACGAGCCCCTCAGGGGGGAG
  GTCGCCACCTCTGTGGACCTGATTACACTGCACAATCGGGGCTGGCGGATTAGGATTGTGCCTGATGAGC
  TTACGACCATATTTCCCGAATGGAAGTGCGTGGCGAGGGAGTACGTCCAGCTGAACATCACAGCCAAAGA
  GCGCGCAGATAAAGAGAAGAACCAGACCATGCGCAGCATTGCTAAACTCCTTTCAAATGCCCTCTATGGC
  AGTTTTGCCACAAAGCTGGACAACAAAAAAATCGTGTTTAGTGATCAGATGGACGAGAGCCTGATGAAGG
  GAATTAGCGCAGGCACAGTAAATATAAAAAGTTCCAGCTTTCTGGAAACAGATAACCTGAGCGCAGAGGT
  AATGCCCGCCTTCGAGAGAGAATACCTTCCGCAGCAGCTCGCCCTGCTTGATAGCGACCCTGAAGACTCA
  GAGGATGAGCAGCGGCCAGCCCCGTTTTATACCCCACCGGCTGGAACACCTGGACACGTTGCATACACTT
  ACAAACCTATAACCTTCCTGGATGTGGAAGAGGGTGACATGTGTTTGCACACAGTAGAGAAAGTCGATCC
  TCTTGTGGACAACGACAGGTACCCAAGTCACGTAGCTAGCTTCGTCCTGGCCTGGACGCGGGCCTTTGTC
  AGCGAATGGGCCGGTTTTCTCTATGAAGAAGATCGCGGCACACCACTTGAGGACAGGCCAATAAAATCAG
  TGTACGGCGACACCGATAGCCTGTTTGTGACACAGAGAGGACATGAACTCATGGAGACAAAAGGCAAGAA
  GCGGATCAAGAAACATGGCGGAAAACTTGTTTTTGATCCAGATGAGCCTGATCTTACATGGCTCGTCGAG
  TGCGAGACCGTCTGTGCGAGCTGCGGCGCAGACGCTTACTCCCCTGAGAGCATCTTCTTGGCCCCCAAGC
  TTTACGCCCTCAAATGTATTTATTGTCCCGCTTGTCACAAAACGAGCAAAGGCAAACTTAGAGCTAAGGG
  ACACGCGGCCGAGGCCTTGAATTACGAGTTGATGGTGAATTGCTACCTGGCCGACATGCAGGGAGCCAAT
  CGGCAGCGCTTCTCCACCTCTCGCATGTCTCTGAAAAGGACCTTGGCCAGCGCCCAACCAGGAGCTCACC
  CTTTTACCGTGACTGAGACAACCCTGACACGCACACTGAGACCTTGGAAGGATCGGACTTTGGCAGCACT
  GGACGCACATAGGCTGATCCCGTATTCCCGGAGCCGGCCGAATCCAAGAAATGAAGAGGTGTGTTGGATC
  GAGATGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100333 representing YP_002213831
  Red=Cloning sites Green=Tags
  
  MALVQAHGAGSFHPEASDPGCQPPRRRACESSQGAVPEPTRARRRRTTASHAAGSRAAPAARCAPSTPLL
  NMQEATEPPPSKMKNKGTVVAPKGHGTLQAIDISTNGPVEIKYYLNLPHALQKIMQVNLLTLPTNLTPQR
  LRTLDSSGLRALVLELRPCRAEVWTCLPRGLVSMTTIETEDGHADADNIVKREVQAPGLNFPLKFLVKGS
  QVQLIHKVHPVNRCEYCGRLYKHKHECSARRREFYFHHINSHSSNWWQEIQFFPIGSHPRTERLFLTYDV
  ETYTWMGSFGKQLIPFMLVMKLSGDQRLVNIAYDLAIKLKWDRWRQDPQTFYCVTPEKMAVGQHFRQYRD
  QLQTALAVDLWSSFLKANPHVHEWALEHYALTDPTDLTFEELKKLPHVRGTPRFLELYIVGHNINGFDEI
  VLAAQVINNRAEVPQPFKITRNFMPRAGKILFNDVTFALPNPAYKKRVDFQLWEQGACDDIDFKYQFLKV
  MVRDTFALTHTSLRNAAQAYSLPVEKGCCPYKAVNQFYMLGSYRAEKDGFPLEEYWKDHEEYLLNRELWE
  KKSQPRYDIIQETLDYCALDVLITAELVAKLQESYAHFIRDSVGLPHAHFNIFQRPTISSNSHAIFRQIV
  YRAEKPNRTNLGPGLLAPSHELYDYVRASIRGGRCYPTYIGILEEPLYVYDICGMYASALTHPMPWGTPL
  NPYERALAVREWQMTLDDPATISYFDKDLLPGIFTIDADPPDEFMLDPLPPFCSRKGGRLCWTNEPLRGE
  VATSVDLITLHNRGWRIRIVPDELTTIFPEWKCVAREYVQLNITAKERADKEKNQTMRSIAKLLSNALYG
  SFATKLDNKKIVFSDQMDESLMKGISAGTVNIKSSSFLETDNLSAEVMPAFEREYLPQQLALLDSDPEDS
  EDEQRPAPFYTPPAGTPGHVAYTYKPITFLDVEEGDMCLHTVEKVDPLVDNDRYPSHVASFVLAWTRAFV
  SEWAGFLYEEDRGTPLEDRPIKSVYGDTDSLFVTQRGHELMETKGKKRIKKHGGKLVFDPDEPDLTWLVE
  CETVCASCGADAYSPESIFLAPKLYALKCIYCPACHKTSKGKLRAKGHAAEALNYELMVNCYLADMQGAN
  RQRFSTSRMSLKRTLASAQPGAHPFTVTETTLTRTLRPWKDRTLAALDAHRLIPYSRSRPNPRNEEVCWI
  EMP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 3579 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213831
• RefSeq ORF: 3579
• Locus ID: 6870517
• MW: 135.9 kDa