E3 (NC_011203) Virus Tagged ORF Clone

CAT#: VC100354

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-gamma [Human adenovirus B], codon optimized for human cell expression, YP_002213790

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100354 represents NCBI reference of YP_002213790 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTTCCACTACAATTTTTATCATAACCAGCCTCGCTGCTGTGACGTATGGTAGAAGCCACCTCACAG
  TGCCCGTGGGATCCACATGTACCCTCCAAGGCCCACAGGAGGGTTATGTGACCTGGTGGCGGATCTATGA
  CAACGGTGGGTTTGCACGCCCCTGCGATCAGCCAGGGACTAAATTCTCCTGTAACGGACGGGACCTTACA
  ATAATTAACATAACTTCTAACGAACAGGGGTTTTACTACGGAACTAACTACAAAAATAGTCTGGATTACA
  ACATTATCGTTGTACCAGCAACAACCTCCGCCCCTCGGAAATCTACATTCTCTTCCTCCTCTGCCAAGGC
  TTCCACAATTCCAAAGACCGCTTCTGCGATGCTTAAGCTGCCCAAGATTGCCCTCTCAAATAGTACTGCC
  GCACCCAACACCATTCCCAAGTCCACAATCGGCATAATCACCGCCGTGGTGGTGGGCTTGATGATCATCT
  TCCTGTGCATTATGTATTATGCGTGCTGCTATCGGAAACACGAACAGAAAGGAGACGCACTGCTCAACTT
  TGACATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100354 representing YP_002213790
  Red=Cloning sites Green=Tags
  
  MISTTIFIITSLAAVTYGRSHLTVPVGSTCTLQGPQEGYVTWWRIYDNGGFARPCDQPGTKFSCNGRDLT
  IINITSNEQGFYYGTNYKNSLDYNIIVVPATTSAPRKSTFSSSSAKASTIPKTASAMLKLPKIALSNSTA
  APNTIPKSTIGIITAVVVGLMIIFLCIMYYACCYRKHEQKGDALLNFDI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 567 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213790
• RefSeq ORF: 567
• Locus ID: 6870528
• MW: 20.5 kDa