L3 (NC_011203) Virus Tagged ORF Clone

CAT#: VC100344

Myc-DDK-tagged ORF clone of viral ORF for protease [Human adenovirus B], codon optimized for human cell expression, YP_002213780

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100344 represents NCBI reference of YP_002213780 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTGCGGCTCAGGCAACGGGTCTAGCGAACAGGAGCTGAAAGCTATCGTTCGCGACCTGGGCTGTG
  GACCCTATTTCCTTGGCACATTCGATAAGAGATTCCCCGGCTTCATGGCACCAGACAAGCTGGCATGCGC
  CATTGTTAATACCGCAGGGCGGGAGACAGGTGGCGAGCACTGGCTGGCCTTTGGATGGAACCCCCGGTAC
  AATACGTGTTACCTCTTTGACCCTTTTGGCTTCTCCGACGAACGCCTTAAGCAGATTTACCAATTCGAGT
  ACGAGGGACTTTTGCGCCGCTCAGCGCTCGCAACAAAAGACCGGTGCATTACGTTGGAGAAATCAACACA
  GTCCGTCCAGGGTCCTAGATCCGCAGCATGCGGATTGTTTTGTTGCATGTTCCTTCATGCCTTTGTGCAC
  TGGCCAGACCGCCCTATGGATGGCAATCCGACTATGAAACTGCTGACTGGGGTCAGCAACTCTATGCTGC
  AGTCACCCCAGGTCCAACCAACCTTGAGGCGGAATCAGGAGGTGCTGTATCGGTTTCTCAATACCCACTC
  CTCCTACTTTAGATCCCACAGAGCCCGAATCGAGCGGGCCACCGCGTTTGATAGGATGGACATGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100344 representing YP_002213780
  Red=Cloning sites Green=Tags
  
  MTCGSGNGSSEQELKAIVRDLGCGPYFLGTFDKRFPGFMAPDKLACAIVNTAGRETGGEHWLAFGWNPRY
  NTCYLFDPFGFSDERLKQIYQFEYEGLLRRSALATKDRCITLEKSTQSVQGPRSAACGLFCCMFLHAFVH
  WPDRPMDGNPTMKLLTGVSNSMLQSPQVQPTLRRNQEVLYRFLNTHSSYFRSHRARIERATAFDRMDMQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 627 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213780
• RefSeq ORF: 627
• Locus ID: 6870521
• MW: 23.8 kDa