E3 (NC_011203) Virus Tagged ORF Clone

CAT#: VC100357

Myc-DDK-tagged ORF clone of viral ORF for membrane protein E3 RID-beta [Human adenovirus B], codon optimized for human cell expression, YP_002213793

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100357 represents NCBI reference of YP_002213793 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAGCTATGCTGCCGGTAATCTTGATTCTGCTTCTGCCCTGTATTCCGCTGGCCAGTACTGCAACCA
  GGGCCACCCCCGAGCAGTTGAGAAAGTGTAAGTTCCAGCAACCATGGTCCTTCCTGGACTGCTACCACGA
  AAAATCTGACTTTCCTACATATTGGATCGTGATTGTGGGGATTATCAACATTCTGTCTTGTACTTTTTTC
  TCCATTACTATCTACCCGACATTCAATTTCGGCTGGAACAGCCCTAACGCTCTGGGCTACCCCCAAGAAC
  CCGACGAACATATACCTCTTCAGCACATCCAGCAGCCGCTGGCTCTCGTGCAGTATGAGAACGAGCCACA
  ACCCTCACTCCCACCAGCAATTTCTTACTTTAATCTGACCGGTGGGGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100357 representing YP_002213793
  Red=Cloning sites Green=Tags
  
  MQAMLPVILILLLPCIPLASTATRATPEQLRKCKFQQPWSFLDCYHEKSDFPTYWIVIVGIINILSCTFF
  SITIYPTFNFGWNSPNALGYPQEPDEHIPLQHIQQPLALVQYENEPQPSLPPAISYFNLTGGDD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 402 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213793
• RefSeq ORF: 402
• Locus ID: 6870528
• MW: 15.2 kDa