E4 (NC_011203) Virus Tagged ORF Clone

CAT#: VC100364

Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf3 [Human adenovirus B], codon optimized for human cell expression, YP_002213838

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100364 represents NCBI reference of YP_002213838 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAGTCTGTCTGCGGATGGTAGTGGAAGGCGCCTTGAGGGACCTGTTCGTGATGTGCGGCCTTGACC
  TGCCCCAGGAATTGACAAGGATTATCCAGGGATGGAAGGCCGAAAATTACCTTGGGATGGTGCAAGAGTG
  CAATATGATGTTCGAGGAACTGGAAAACGCTCCGTCCTTCGGAATCCTTCTTTTCTTGGACGTAAGAGTG
  GAGGCCTTGTTGGAAGCAACCGTCGAGCACTTGGAAAACCGCATTAGCTTTGATCTCGCAGTACTTTTCC
  ACCAGCATTCTGGAGGTGACCGATGCCACCTGCGAGATCTCCAGTTTGAAGTCCTCCGGGACAGACTTGA
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100364 representing YP_002213838
  Red=Cloning sites Green=Tags
  
  MRVCLRMVVEGALRDLFVMCGLDLPQELTRIIQGWKAENYLGMVQECNMMFEELENAPSFGILLFLDVRV
  EALLEATVEHLENRISFDLAVLFHQHSGGDRCHLRDLQFEVLRDRLE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 351 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213838
• RefSeq ORF: 351
• Locus ID: 6870530
• MW: 13.6 kDa