L1 (NC_011203) Virus Tagged ORF Clone

CAT#: VC100336

Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein 52K [Human adenovirus B], codon optimized for human cell expression, YP_002213772

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100336 represents NCBI reference of YP_002213772 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCCTGTTCTGAGACAGATGCGCCCCCAGCAGCAGGCACCATCTCAGCAGCAACCACAAAAGGCAC
  TGTTGGCCCCCGCGACCACTGCTGCCGCAGCCGTGTCCGGAGCAGGGCAGCCAGCTTACGACCTGGACCT
  TGAGGAAGGGGAGGGACTCGCAAGGCTGGGAGCTCCCTCCCCTGAGCGGCACCCCAGGGTGCAGCTCAAG
  AAAGATTCTAGGGAAGCATACGTTCCCCAGCAGAACTTGTTCCGCGACCGATCCGGCGAAGAACCGGAAG
  AGATGAGAGCGTCCAGGTTTAACGCAGGGCGGGAGCTTAGACACGGACTCGACCGACGACGGGTGCTCCA
  AGACGAGGATTTCGAGGTAGACGAGGTTACCGGTATTTCACCCGCTAGAGCCCACGTGGCTGCTGCCAAC
  CTTGTGAGCGCCTACGAACAGACCGTTAAGGAGGAGAGAAACTTCCAGAAATCATTTAATAATCACGTGC
  GCACACTGATCGCCAGAGAGGAGGTGACCCTTGGTCTGATGCACCTTTGGGACCTCATGGAAGCGATAGC
  CCAGAATCCCACAAGCAAACCCCTCACCGCCCAGTTGTTTCTGGTCGTGCAGCATTCTCGGGATAACGAA
  GCTTTCAGGGAGGCTCTGCTCAATATCACCGAACCTGATGGCAGATGGCTCTACGACCTGATCAACATTC
  TGCAATCAATTATCGTGCAGGAACGGTCTCTGGGACTCGCAGAGAAAGTGGCGGCTATCAATTACAGTGT
  TCTCTCCCTCGGCAAGTATTACGCACGGAAGATTTACAAGACGCCCTATGTTCCGATTGACAAGGAAGTG
  AAGATCGATGGATTTTACATGAGAATGACCTTGAAGGTCCTGACCTTGAGCGATGACCTGGGGGTTTATA
  GGAACGACCGGATGCACCGCGCCGTGTCAGCAAGTCGGCGCCGAGAGCTCAGCGACCGAGAATTGATGCA
  CTCACTGCAGCGGGCACTCACCGGTGCCGGCACTGATGGCGAGAACTATTTCGATATGGGGGCCGACCTT
  CAGTGGCAGCCTAGCCGCCGAGCTATGGAAGCCGCCGGATGCGAGCTGCCATACATCGAGGAAGTTGATG
  AAGTCGAAGATGAGGAGGGGGAGTACCTGGAGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100336 representing YP_002213772
  Red=Cloning sites Green=Tags
  
  MHPVLRQMRPQQQAPSQQQPQKALLAPATTAAAAVSGAGQPAYDLDLEEGEGLARLGAPSPERHPRVQLK
  KDSREAYVPQQNLFRDRSGEEPEEMRASRFNAGRELRHGLDRRRVLQDEDFEVDEVTGISPARAHVAAAN
  LVSAYEQTVKEERNFQKSFNNHVRTLIAREEVTLGLMHLWDLMEAIAQNPTSKPLTAQLFLVVQHSRDNE
  AFREALLNITEPDGRWLYDLINILQSIIVQERSLGLAEKVAAINYSVLSLGKYYARKIYKTPYVPIDKEV
  KIDGFYMRMTLKVLTLSDDLGVYRNDRMHRAVSASRRRELSDRELMHSLQRALTGAGTDGENYFDMGADL
  QWQPSRRAMEAAGCELPYIEEVDEVEDEEGEYLED
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 1155 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213772
• RefSeq ORF: 1155
• Locus ID: 6870523
• MW: 43.7 kDa