E3 (NC_011203) Virus Tagged ORF Clone

CAT#: VC100355

Myc-DDK-tagged ORF clone of viral ORF for membrane protein E3 CR1-delta [Human adenovirus B], codon optimized for human cell expression, YP_002213791

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100355 represents NCBI reference of YP_002213791 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCTGTTTCAGAGTAACACTACGACAAGCTACGCTTACACGAACATCCAGCCTAAATATGCCATGC
  AGCTTGAGATCACAATTCTTATCGTTATCGGCATTCTGATCCTGAGCGTTATCCTTTATTTCATTTTTTG
  TCGGCAGATCCCAAACGTTCATCGCAATTCAAAAAGACGCCCAATTTATTCCCCTATGATAAGCCGGCCC
  CATATGGCCCTGAATGAAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100355 representing YP_002213791
  Red=Cloning sites Green=Tags
  
  MILFQSNTTTSYAYTNIQPKYAMQLEITILIVIGILILSVILYFIFCRQIPNVHRNSKRRPIYSPMISRP
  HMALNEI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 231 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213791
• RefSeq ORF: 231
• Locus ID: 6870528
• MW: 9.0 kDa