L3 (NC_011203) Virus Tagged ORF Clone

CAT#: VC100342

Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pVI [Human adenovirus B], codon optimized for human cell expression, YP_002213778

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100342 represents NCBI reference of YP_002213778 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACATCAATTTCTCTTCACTGGCCCCTCGCCACGGGACTAGGCCCTATATGGGAACCTGGTCTG
  ACATAGGAACTAGTCAGCTGAACGGAGGCGCCTTTAATTGGAGCAGCATATGGTCCGGTCTGAAGAACTT
  CGGGAGCACTGTTAAGACTTACGGGAACAAAGCCTGGAATAGCTCCACAGGACAAGCCCTCCGAAATAAA
  TTGAAGGAACAAAACTTCCAGCAGAAAGTTGTGGATGGTATCGCTTCCGGCATTAACGGCGTAGTGGACC
  TGGCAAATCAGGCTGTGCAAAAACAAATAAATTCCCGCCTGGACCCCCCACCTGCGACACCTGGCGAAAT
  GGAAGTCGAGGAGGAGCTGCCCCCACTGGAGAAACGCGGGGACAAACGACCAAGGCCCGAACTCGAACAG
  ACACTTGTGACGAGAGCCGACGAGCCACCCAGCTACGAGGAGGCCGTAAAACTTGGCATGCCCACTACTC
  GGCCTGTAGCCCATATGGCGACTGGTGTGATGAAGCCATCTCAATCACACCGCCCAGCGACACTCGACTT
  GCCGCCCCCACCCGCTTCAGCCGCACCGGTGCCTAAACCTGTGGCTACCAGGAAGCCTACAGCCGTACAA
  CCCGTAGCAGTGGCCCGGCCAAGACCAGGCGGAACTCCCCGGCCCAAAGCGAACTGGCAGTCAACGCTGA
  ACAGTATTGTAGGACTGGGAGTACAATCCGTAAAGCGCCGCCGGTGTTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100342 representing YP_002213778
  Red=Cloning sites Green=Tags
  
  MEDINFSSLAPRHGTRPYMGTWSDIGTSQLNGGAFNWSSIWSGLKNFGSTVKTYGNKAWNSSTGQALRNK
  LKEQNFQQKVVDGIASGINGVVDLANQAVQKQINSRLDPPPATPGEMEVEEELPPLEKRGDKRPRPELEQ
  TLVTRADEPPSYEEAVKLGMPTTRPVAHMATGVMKPSQSHRPATLDLPPPPASAAPVPKPVATRKPTAVQ
  PVAVARPRPGGTPRPKANWQSTLNSIVGLGVQSVKRRRCY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 750 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213778
• RefSeq ORF: 750
• Locus ID: 6870521
• MW: 27.1 kDa