L4 (NC_011203) Virus Tagged ORF Clone

CAT#: VC100347

Myc-DDK-tagged ORF clone of viral ORF for protein 33K [Human adenovirus B], codon optimized for human cell expression, YP_002213783

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100347 represents NCBI reference of YP_002213783 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACACAGAAGGGGCTCCAAAAAGCTCAAAGTCCAGCTGAGCCCACCGGAAGACATGAAAGAGGACT
  GGGACAGCCAAGCCGAGGAAGAGATGGAAGATTGGGATTCACAGGCAGAAGAAGTCGATTCCTTGGAGGA
  GGATTCTCTGGAAGAGGAGGATGAAGAGGCTGAAGAAGTAGAGGAGGCTACTGCCGCAAAACAGCTGAGC
  TCTGCAGCCGAAACTAGTAAAAGTCCTGACTCTTCCACAGCAACTATCTCAGCGCCTGGAAGGGGGGCTC
  AGCGCCGGCCAAATAGCCGGTGGGACGAGACAGGCCGCTTCCCCAATCCAACAACTGCCTCCAAGACTGC
  CCCAACAGCAGTTTCAAAATCACGCCAGCCACGCCAAAAAAACACCGCTGCCACGACAGTTACTCGGAAA
  CCCGCAGTCCGAAAGTCCACTACTTCAGCCCCTGGCGGGGGTCTGAGAATTACCGCAAACGAACCTGCTC
  AGACGCGGGAGCTCAGGAACAGGATCTTTCCTACCCTTTATGCCATTTTCCAGCAATCCCGGGGCCAAGA
  GCAGGAGTTGAAGGTCAAGAATAGGTCTCTGCGGAGCCTCACCAGATCATGTCTGTATCATAAATCTGAA
  GATCAGCTGCAGAGAACACTCGAGGACGCCGAAGCTCTGTTTAACAAGTACTGTGCCCTCACTTTGAAGG
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100347 representing YP_002213783
  Red=Cloning sites Green=Tags
  
  MSHRRGSKKLKVQLSPPEDMKEDWDSQAEEEMEDWDSQAEEVDSLEEDSLEEEDEEAEEVEEATAAKQLS
  SAAETSKSPDSSTATISAPGRGAQRRPNSRWDETGRFPNPTTASKTAPTAVSKSRQPRQKNTAATTVTRK
  PAVRKSTTSAPGGGLRITANEPAQTRELRNRIFPTLYAIFQQSRGQEQELKVKNRSLRSLTRSCLYHKSE
  DQLQRTLEDAEALFNKYCALTLKE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 702 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213783
• RefSeq ORF: 702
• Locus ID: 6870520
• MW: 26.2 kDa