L4 (NC_011203) Virus Tagged ORF Clone

CAT#: VC100346

Myc-DDK-tagged ORF clone of viral ORF for hexon assembly protein 100K [Human adenovirus B], codon optimized for human cell expression, YP_002213782

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100346 represents NCBI reference of YP_002213782 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACACAGCCTAGTCTGCCTACCAGCCTGCAAGCACCCTCACATCTGGCCCCCTCATCCGACGAGG
  AGGAGTCTCTGACTACGCCTCCCCCTAGCCCAGCTACGACCACGTCCACCCTGGACGATGAGGAGGAGGT
  CGACGCTGCCCAGGAAATGCAGGCACAAGACAACGTTAAGACAGAGGAGATCGAGGCTGACGTTGAGCAG
  GATCCGGGATATGTGACACCGGCCGAACACCAGGAGGAGCTGAAACGCTTTCTGGATAGAGAGGACGACG
  ATAGGCCCGAGCACCAGGCCGATGGCGTCTATCAAGAAGCCGGAATCAGAGACCACGTGGCGGACTACCT
  GACGGGTCTGGGGGGTGAGGACGTTCTGCTGAAACATCTCGCTAGACAAAGTATCATTGTGAAGGACGCT
  CTGTTGGACCTCACCGAGGTGCCTATTAGCGTTGAGGAACTGTCCAGGGCCTACGAACTGAACCTTTTCA
  GCCCTCAGGTGCCCCCTAAGAGGCAGCCCAATGGCACATGCGAGGCTAACCCACGACTCAACTTCTACCC
  AGCCTTTACTATTCCCGAGGTTCTGGCAACGTACCACATATTCTTCAAGAACCAGAAGATTCCAGTCAGC
  TGTAGAGCCAATAGAACAAGAGCCGATGCTCTGTTGAACCTTGGACCTGGGGCCAGATTGCCAGACATTG
  CATCTTTGGAGGAAGTGCCGAAGATATTCGAGGGTCTGGGCTCAGATGAAACCAGAGCAGCAAATGCACT
  GCAACAAGGAGAGAACGACATGGATGAGCATCACAGTGCACTTGTGGAGCTCGAGGGAGACAATGCTAGG
  ATCGCAGTTCTGAAGCGCTCCATCGTGGTGACACATTTCGCTTATCCTGCAGTTAACCTGCCTCCCAAAG
  TTATGTCTGCTGTCATGGATCACCTGCTTATTAAGCGCGCGCGGCCACTGAGTGAGAATCAGAACATGCA
  GGATCCGGATGCTTCCGATGAAGGTAAACCTGTGGTCTCTGATGAACAGCTGTCAAGGTGGCTTGGAACT
  AATTCCCCTAGAGATCTTGAGGAGCGGCGAAAACTTATGATGGCTGTCGTTTTGGTCACCGTGGAGCTCG
  AATGCCTCCGCAGGTTTTTTACCGACCCAGAGACCCTCCGCAAACTTGAAGAAAATCTTCACTACACTTT
  CCGCCACGGATTCGTGCGCCAAGCCTGCAAGATTTCTAACGTCGAGCTGACTAATCTGGTGTCTTACATG
  GGTATCCTGCACGAGAACAGACTCGGCCAATCAGTACTGCATACGACACTGAAGGGCGAGGCACGCCGGG
  ATTATATCAGGGACTGCGTATATTTGTACCTTTGTCATACCTGGCAGACCGGCATGGGGGTATGGCAGCA
  GTGTCTGGAAGAGCAAAATCTGAAAGAGCTTGATAAACTTCTCCAACGGTCTTTGAAGGCCTTGTGGACT
  GGATTCGATGAACGCACAGTGGCATCAGACCTGGCTGATATTATATTTCCTGAACGGCTGCGGGTTACGC
  TGCGGAACGGCCTCCCCGACTTCATGTCACAGAGTATGCTCAACAATTTCCGGAGCTTTATACTGGAAAG
  ATCTGGGATCCTGCCCGCCACTTGCTGCGCCCTCCCCTCTGATTTTGTGCCACTGACCTACAGAGAATGC
  CCACCCCCTCTGTGGTCACACTGCTATCTCTTCCGGCTGGCCAATTATCTGAGCTATCACTCTGATGTGA
  TTGAAGACGTGTCTGGGGATGGATTGCTGGAGTGCCACTGCCGGTGCAACCTCTGTACACCTCATAGGTC
  TCTTGCGTGCAATCCCCAGCTGCTGAGTGAAACACAGATTATAGGAACTTTTGAGCTGCAAGGACCCAGA
  TCCAAAGGGGAGGGAAGCAGTCCCGGCCAAAGCCTTAAACTTACTCCTGGGCTGTGGACAAGTGCCTATC
  TGCGGAAATTCCACCCGGAAGATTACCACCCATATGAAATCCGCTTTTATGAGGACCAGAGTCACCCACC
  CAAAGTGGAGCTTTCAGCATGCGTTATCACCCAGGGAGCCATCCTTGCACAGCTGCAGGCAATCCAGAAG
  TCTAGGCAGGAGTTCCTCCTCAAAAAGGGTAATGGCGTGTACCTCGATCCACAGACTGGTGAAGAGCTCA
  ACACTAGGTTCCCACAGGACGTGCCTAGCCCACGGAAGCAGGAGGTGGAAGGCGCTGCTGTTGCTCCCAG
  GGGGTACGAGGGGAGACTCGGACAATCCGGTAGAGGTGGTGATGGTCGACTTGGGCAACCTGGGAGAGGG
  GGGGGCCAGCCTGGAGGCAGACAATTTGGAGGGGGTAGGCGCGGTGGAAGAGGAGGTGGACGATCAAACC
  GCAGGCAGACGGTGGTCATTGGCGGCGGGGATAAACAGGTGCCAAGGCAGCAACATGGATATCATCTCCG
  GTCCGGCTCTGGCGGACCAGCTGCTGCCCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100346 representing YP_002213782
  Red=Cloning sites Green=Tags
  
  METQPSLPTSLQAPSHLAPSSDEEESLTTPPPSPATTTSTLDDEEEVDAAQEMQAQDNVKTEEIEADVEQ
  DPGYVTPAEHQEELKRFLDREDDDRPEHQADGVYQEAGIRDHVADYLTGLGGEDVLLKHLARQSIIVKDA
  LLDLTEVPISVEELSRAYELNLFSPQVPPKRQPNGTCEANPRLNFYPAFTIPEVLATYHIFFKNQKIPVS
  CRANRTRADALLNLGPGARLPDIASLEEVPKIFEGLGSDETRAANALQQGENDMDEHHSALVELEGDNAR
  IAVLKRSIVVTHFAYPAVNLPPKVMSAVMDHLLIKRARPLSENQNMQDPDASDEGKPVVSDEQLSRWLGT
  NSPRDLEERRKLMMAVVLVTVELECLRRFFTDPETLRKLEENLHYTFRHGFVRQACKISNVELTNLVSYM
  GILHENRLGQSVLHTTLKGEARRDYIRDCVYLYLCHTWQTGMGVWQQCLEEQNLKELDKLLQRSLKALWT
  GFDERTVASDLADIIFPERLRVTLRNGLPDFMSQSMLNNFRSFILERSGILPATCCALPSDFVPLTYREC
  PPPLWSHCYLFRLANYLSYHSDVIEDVSGDGLLECHCRCNLCTPHRSLACNPQLLSETQIIGTFELQGPR
  SKGEGSSPGQSLKLTPGLWTSAYLRKFHPEDYHPYEIRFYEDQSHPPKVELSACVITQGAILAQLQAIQK
  SRQEFLLKKGNGVYLDPQTGEELNTRFPQDVPSPRKQEVEGAAVAPRGYEGRLGQSGRGGDGRLGQPGRG
  GGQPGGRQFGGGRRGGRGGGRSNRRQTVVIGGGDKQVPRQQHGYHLRSGSGGPAAAQQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 2484 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213782
• RefSeq ORF: 2484
• Locus ID: 6870520
• MW: 92.3 kDa