E1B (NC_011203) Virus Tagged ORF Clone

CAT#: VC100329

Myc-DDK-tagged ORF clone of viral ORF for control protein E1B 19K [Human adenovirus B], codon optimized for human cell expression, YP_002213766

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100329 represents NCBI reference of YP_002213766 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGTCTGGGCCATTCTCGAGGATCTGAGACAAACCAAACTGCTTTTGGAAAATGCAAGCGATGGCG
  TGAGTGGGCTCTGGCGATTCTGGTTTGGGGGAGATCTCGCCCGGCTGGTGTTTCGCATAAAACAAGACTA
  CCGCGAGGAGTTCGAGAAACTCCTCGACGATTCACCTGGTCTGTTCGAGGCATTGAATTTGGGACACCAG
  GCCCACTTTAAAGAAAAAGTCCTCTCAGTCCTCGATTTTTCTACACCTGGAAGGACCGCGGCGGCTGTCG
  CTTTTCTGACTTTCATCCTGGACAAGTGGATCCGACAAACTCATTTTAGTAAGGGTTACGTGCTCGACTT
  CATCGCCGCGGCGCTGTGGCGGACATGGAAGGCCAGGCGCATGAGAACTATACTGGATTATTGGCCTGTT
  CAGCCCCTGGGGGTCGCAGGCATACTGCGACACCCCCCCACCATGCCAGCAGTGCTCCAAGAAGAGCAAC
  AGGAAGATAACCCAAGGGCGGGCCTGGACCCACCAGTGGAGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100329 representing YP_002213766
  Red=Cloning sites Green=Tags
  
  MEVWAILEDLRQTKLLLENASDGVSGLWRFWFGGDLARLVFRIKQDYREEFEKLLDDSPGLFEALNLGHQ
  AHFKEKVLSVLDFSTPGRTAAAVAFLTFILDKWIRQTHFSKGYVLDFIAAALWRTWKARRMRTILDYWPV
  QPLGVAGILRHPPTMPAVLQEEQQEDNPRAGLDPPVEE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 534 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213766
• RefSeq ORF: 534
• Locus ID: 6870515
• MW: 20.5 kDa