IVa2 (NC_011203) Virus Tagged ORF Clone

CAT#: VC100332

Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein IVa2 [Human adenovirus B], codon optimized for human cell expression, YP_002213830

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IVa2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100332 represents NCBI reference of YP_002213830 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACTAAGGGCAGGCAGAGTGCAGCTGTCTTGGACCAACAGGATGAATCCGAAGCTCACCCACGCA
  AGAGACCTACGAGACGCGCACCTCTCCACAGGGATGGGAACAACCCCGATAGTGATGCCGCGACTATGGA
  AGGTAGTGATCCGGGCAGCGCAGGGAGGCCATCAAGCGACTCATTGCTGCAGGAACCAAGTCAACCAGCC
  AAGAGAGGCGGACTGCTGGATCGCGATGCAATCGAACATATCACTGAACTTTGGGATAGACTGGAACTGC
  TGCAGCAGACTCTCTCAAAGATGCCTATGGCTGATGGGCTGAAGCCATTGAAGAATTTCAGCAGCCTCCA
  AGAATTGTTGTCACTGGGAGGTGAGAGACTCCTTACCGACCTTGTCCGGGAGAATATACACGTTAGAGAG
  ATGATGAATGAGGTCGCTCCATTGCTTAGGGAGGATGGCTCCTGCAGGTCTCTTAATTATCACCTCCAGC
  CTGTCATCGGTGTGATCTACGGTCCCACAGGCTGCGGGAAGAGTCAACTGCTGCGGAACCTTTTGAGTTC
  ACAGCTCATAACACCAGCACCCGAGACTGTATTTTTCATTGCCCCTCAGGTGGATATGATTCCACCCAGC
  GAGCTGAAAGCATGGGAGATGCAAATTTGTGAGGGGAATTACGCTCCCGGGCCAGAGGGAACCTTTATAC
  CCCAGTCAGGAACCTTGCGGCCTAAGTTTATCAAAATGGCCTACGATGATCTCACACAGGAGCATAACTA
  TGATGTTTCAGACCCTCGGAACGTGTTCGCCAGGGCCGCTGCCCACGGTCCCATCGCAATCATCATGGAT
  GAATGCATGGAGAACCTTGGGGGCCACAAAGGTGTGAGCAAATTTTTCCATGCCTTCCCCTCTAAGCTGC
  ACGACAAGTTTCCCAAGTGCACTGGCTACACTGTCCTGGTAGTGCTCCATAATATGAATCCCCGCCGGGA
  TCTGGGTGGCAACATCGCTAATCTTAAGATCCAGTCCAAAATGCATATAATTAGCCCCAGGATGCATCCC
  AGTCAACTGAATCGATTCGTGAACACATATACAAAAGGTTTGCCTGTGGCTATCAGCCTGCTTTTGAAAG
  ACATTGTGCAGCATCACGCTTTGCGCCCCTGTTACGACTGGGTGATCTATAATACTACTCCAGAGCAGGA
  GGCACTCCAATGGTCCTACCTTCATCCACGCGACGGCTTGATGCCTATGTACTTGAATATACAGTCTCAC
  TTGTATCGCGTGTTGGAGAAGATTCACAGAGTGTTGAACGACAGAGATAGGTGGTCCAGGGCCTACCGGG
  CGAGGAAGATTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100332 representing YP_002213830
  Red=Cloning sites Green=Tags
  
  METKGRQSAAVLDQQDESEAHPRKRPTRRAPLHRDGNNPDSDAATMEGSDPGSAGRPSSDSLLQEPSQPA
  KRGGLLDRDAIEHITELWDRLELLQQTLSKMPMADGLKPLKNFSSLQELLSLGGERLLTDLVRENIHVRE
  MMNEVAPLLREDGSCRSLNYHLQPVIGVIYGPTGCGKSQLLRNLLSSQLITPAPETVFFIAPQVDMIPPS
  ELKAWEMQICEGNYAPGPEGTFIPQSGTLRPKFIKMAYDDLTQEHNYDVSDPRNVFARAAAHGPIAIIMD
  ECMENLGGHKGVSKFFHAFPSKLHDKFPKCTGYTVLVVLHNMNPRRDLGGNIANLKIQSKMHIISPRMHP
  SQLNRFVNTYTKGLPVAISLLLKDIVQHHALRPCYDWVIYNTTPEQEALQWSYLHPRDGLMPMYLNIQSH
  LYRVLEKIHRVLNDRDRWSRAYRARKIK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213830
• RefSeq ORF: 1344
• Locus ID: 6870518
• MW: 50.7 kDa