E2A (NC_011203) Virus Tagged ORF Clone

CAT#: VC100345

Myc-DDK-tagged ORF clone of viral ORF for single-stranded DNA-binding protein [Human adenovirus B], codon optimized for human cell expression, YP_002213833

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100345 represents NCBI reference of YP_002213833 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCAGGGGTGGAAATCAGAGCAGCGACCGGCACCGCGAGCACACACCCGAGCGCGGCATGTCCA
  GTGCGAGCCATCCCCCTCCCAGACCCGATAGATCACCTTCTCAAAGCCCCCCACCGCTCCCTCCTAAACG
  AAATACATACCGAAGGGTTGGGTCCGGGTCATCCACCGAATCCCAGGTCGTGCTCGTCTCCGAGACAAGC
  CAGAGCAGCCTTTCACCCGAAAGGTCCAATAGCCCGCCCCCGATACCTCCAAAAAAGAAGCCCCGGAAGA
  CTAAGCACGTGCCTTTGCAGGACATTAGTCAAGACTCCGAAGAGGAACGGGAGCAGGCCCAACTGGTGGC
  AGTGGGCTTCAGTTACCCTCCCGTTAGAATTATCGAGAAGGATGGCAAGAGGAGCGTCGAGAAAATTGAC
  AAGAACGATCCCATAGCCAAGGGAGCGACATTTATAGCCGTCAGAAATCCACTGTCCCTGCCACTCGTGT
  CTGCCTGGGAGAAAGGTATGGAGGTTATGGCCGTGCTGATGGAGCGATACAGACTGGACAATGACTTGAG
  AACAAGCTTTAAGCTCATGCCCGAACAGCACGAGCAGTATAAACGCATCTGTCACCAGTATGTGAACGAA
  GAGCATCGGGGGATCCCCCTCACATTCACCAGCATGAAGACACTCACTACCATGATGGGGAGATTTATGC
  AGGGGTTGGTGCATTACTACTCCGAGATCGCACACAACAATTGGGAATGTACAGGTTGTGCCCTTTGGGC
  TCACGGATGTACAGACTATGAAGGGAAGGTCAAGTGTCTTCACGGTACTATCATGATTCAGAAGGATCAC
  ATCATAGAGATGGACGTTGCATCAGAAAACGGACAGAGGGCAATGAAAGAGAACCCGGACAGGGCCAAAA
  TTACCCAAAACAGATGGGGCAGAAATGTGGTGCAGTTGGCTAATAACGACGCCCGGTGTTGTGTTAATGA
  CGCGAACTGTACGACTAACCAGTTCAGCTCTAAGTCCTGTGGCATGTTCTATACAGAAGGTTCAAAGGCA
  CAGGAGGCCTTCAAGCAGTATGAAGCCTTCATGAAGGCCGTGTACCCCGGTATCACACCTGATCAGGCCC
  GAATGATGCTGATTCCGATACATTGTGACTGCAACCACAAGCCGGGTTGCGCCCCCGTGATGGGCCGACA
  GACTTGCAAAATGACACCTTTCGGCATGGCGAACGCCGAAGATTTGGATGTTACTACAATTAGCGATCCA
  ACGGTGCTGGCTTCTGTCCGCCACCCAGCACTTATCGTCTTCCAGTGCTGTAACCCAGTGTATCGGAACA
  GTAGAGTGCAAAATGCAGGCCCTAACTGCGATTTCAAAATCTCTGCTCCTGATCTGCTGGGAGCCATGCA
  GCTCACACGAAAGCTCTGGCAGGACACTTTCCCAGAAATCCCTGTTCCAAAACTTGTGATACCCGAATTC
  AAGTGGCAGAATAGGCTCCAATTCAGGAATGTCAGCCTCCCAGCAGGACACTCTGATAGCCGACAGAACC
  CATTTGATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100345 representing YP_002213833
  Red=Cloning sites Green=Tags
  
  MASRGGNQSSDRHREHTPERGMSSASHPPPRPDRSPSQSPPPLPPKRNTYRRVGSGSSTESQVVLVSETS
  QSSLSPERSNSPPPIPPKKKPRKTKHVPLQDISQDSEEEREQAQLVAVGFSYPPVRIIEKDGKRSVEKID
  KNDPIAKGATFIAVRNPLSLPLVSAWEKGMEVMAVLMERYRLDNDLRTSFKLMPEQHEQYKRICHQYVNE
  EHRGIPLTFTSMKTLTTMMGRFMQGLVHYYSEIAHNNWECTGCALWAHGCTDYEGKVKCLHGTIMIQKDH
  IIEMDVASENGQRAMKENPDRAKITQNRWGRNVVQLANNDARCCVNDANCTTNQFSSKSCGMFYTEGSKA
  QEAFKQYEAFMKAVYPGITPDQARMMLIPIHCDCNHKPGCAPVMGRQTCKMTPFGMANAEDLDVTTISDP
  TVLASVRHPALIVFQCCNPVYRNSRVQNAGPNCDFKISAPDLLGAMQLTRKLWQDTFPEIPVPKLVIPEF
  KWQNRLQFRNVSLPAGHSDSRQNPFDF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 1551 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213833
• RefSeq ORF: 1551
• Locus ID: 6870526
• MW: 58.3 kDa