L4 (NC_011203) Virus Tagged ORF Clone

CAT#: VC100348

Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein 22K [Human adenovirus B], codon optimized for human cell expression, YP_002213784

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100348 represents NCBI reference of YP_002213784 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCATAGGAGAGGTTCAAAGAAATTGAAGGTGCAGCTCTCTCCCCCCGAGGACATGAAGGAAGATT
  GGGATAGCCAGGCTGAAGAAGAGATGGAGGACTGGGATTCCCAAGCCGAAGAGGTGGATTCACTGGAGGA
  GGACAGCCTGGAGGAGGAGGATGAGGAAGCTGAGGAAGTTGAAGAGGCAACAGCCGCGAAGCAGTTGTCA
  TCAGCTGCCGAGACTAGTAAATCTCCAGATAGCTCCACCGCTACTATTTCCGCTCCTGGACGGGGTGCTC
  AACGACGACCAAACAGCAGATGGGACGAAACTGGAAGATTCCCGAATCCCACTACAGCCTCTAAGACAGG
  CAAAAAGGAGCGGCAGGGGTACAAATCCTGGCGAGGCCATAAGAATGCGATCATCAGTTGCCTCCACGAA
  TGCGGGGGAAATATAAGTTTTACCAGGCGCTACCTGTTGTTCCACCACGGTGTGAACTTCCCGAGGAACA
  TTCTCCATTATTACAGGCATCTCCACTCTCCTTATTGCTCCCAGCAGGTTCCTGCAACACCAACGGAGAA
  GTACTCCAGTGACAACGGGGATCAGAAAACCTCCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100348 representing YP_002213784
  Red=Cloning sites Green=Tags
  
  MSHRRGSKKLKVQLSPPEDMKEDWDSQAEEEMEDWDSQAEEVDSLEEDSLEEEDEEAEEVEEATAAKQLS
  SAAETSKSPDSSTATISAPGRGAQRRPNSRWDETGRFPNPTTASKTGKKERQGYKSWRGHKNAIISCLHE
  CGGNISFTRRYLLFHHGVNFPRNILHYYRHLHSPYCSQQVPATPTEKYSSDNGDQKTSS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011203
• ORF Size: 597 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_011203.1, YP_002213784
• RefSeq ORF: 597
• Locus ID: 6870520
• MW: 22.5 kDa