E1A (NC_001454) Virus Tagged ORF Clone

CAT#: VC100500

Myc-DDK-tagged ORF clone of viral ORF for control protein E1A [Human adenovirus F], codon optimized for human cell expression, NP_040845

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100500 represents NCBI reference of NP_040845 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGATGCTTCCTGATTTTTTTACAGGCAATTGGGATGACATGTTCCAGGGACTGCTCGAGACAGAGT
  ACGTGTTCGATTTTCCGGAACCCTCTGAGGCATCTGAAGAAATGTCTCTGCACGACTTGTTCGATGTGGA
  AGTCGATGGCTTCGAGGAAGATGCCAATCAGGAAGCAGTCGATGGTATGTTCCCGGAGAGGCTCCTGTCT
  GAAGCAGAATCTGCAGCAGAGTCTGGCAGTGGAGACAGCGGTGTCGGCGAGGAACTTCTGCCTGTCGATC
  TGGACCTGAAGTGCTATGAGGATGGCTTGCCCCCAAGCGACCCCGAAACTGACGAGGCAACAGAGGCAGA
  GGAAGAAGCCGCAATGCCCACATACGTGAATGAAAATGAGAACGAACTGGTGCTTGACTGCCCTGAGAAC
  CCCGGTCGCGGATGCAGGGCATGTGACTTCCACCGGGGGACTAGTGGGAACCCCGAGGCAATGTGTGCTT
  TGTGCTATATGCGGCTGACCGGGCACTGCATATATTCCCCAATTTCCGATGCCGAGGGTGAGAGCGAATC
  AGGCAGCCCAGAGGACACGGATTTTCCGCACCCCCTTACCGCAACCCCTCCTCACGGCATCGTCCGCACA
  ATTCCTTGCCGCGTCTCTTGTCGCAGGCGGCCCGCCGTGGAGTGCATTGAAGATCTCTTGGAAGAGGACC
  CTACCGACGAACCACTCAATCTTAGCCTGAAGAGGCCTAAGTGTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100500 representing NP_040845
  Red=Cloning sites Green=Tags
  
  MRMLPDFFTGNWDDMFQGLLETEYVFDFPEPSEASEEMSLHDLFDVEVDGFEEDANQEAVDGMFPERLLS
  EAESAAESGSGDSGVGEELLPVDLDLKCYEDGLPPSDPETDEATEAEEEAAMPTYVNENENELVLDCPEN
  PGRGCRACDFHRGTSGNPEAMCALCYMRLTGHCIYSPISDAEGESESGSPEDTDFPHPLTATPPHGIVRT
  IPCRVSCRRRPAVECIEDLLEEDPTDEPLNLSLKRPKCS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 747 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040845
• RefSeq ORF: 747
• Locus ID: 2715915
• MW: 27.5 kDa