E3A (NC_001454) Virus Tagged ORF Clone

CAT#: VC100522

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-beta [Human adenovirus F], codon optimized for human cell expression, NP_040870

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100522 represents NCBI reference of NP_040870 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCTGTTTCTTCTCTGCCTGCTGTTTTGCTCAGCGTATGCGGCCGTCCCAGAAAAGACCCTGAACA
  ATCTGGTCCGAGTTTACGCTCTCGTGGGTACAAACCTCTCCTTGGATAGTATGAAGACCCCACAGATTGA
  CGAACTCACAAGTCTGAGTTGGATTAAGCAAGAAGATAACCCTAACAAGAACTTGCAGTCCTTCTTTTTC
  ATTGGTCAGAAGTTGTGTGAAGTCACAAAGGACAAAATCACTGTGTTCAATTATTACCCGCTGGAATTTA
  GCTGTGCCAATGTGACACTGTATCTGTACAACCTCAAGACAGACGACAGCGGGCTGTATAACGGCAAGGC
  CCATACTAAGGAGCTGGAACACAACACCTACGTGCGCCTTTATGTAATAGATATTCCCCCCCCAAAATGC
  GATATCACCAGTCGCTACTTGGGCATTCAGGCAACTGGCGAAGACTATTGTTTGATAGAAATAAACTGTA
  CTAATTCTAAGTACCCCGCAGTCGTGAAGTTTAATGGTCGGCAATCCAACTTCTACCATTACGTTTCCGA
  AAATGGGAATAAGAAATTGCCTAATTTTTACGAAACCCACATTACGGTCAATGGGACCCACAAAAGCTTT
  CATTTTAATTACCCCTTCAATGACTTGTGCCAGACTACGAGCGCCCTGCAGTACAATGACAACGTGCAGG
  TGGTGCTCATCCTGCTCATCGTCGTCGGGCTTATTATCATATCTGCGTCCCTTATTTTGCTCTACTGTCA
  TCGGAAGAAGATAAAGGCAAAGGTGCAGCACCAACCAGTGCACATTTGTCTGGAAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100522 representing NP_040870
  Red=Cloning sites Green=Tags
  
  MLLFLLCLLFCSAYAAVPEKTLNNLVRVYALVGTNLSLDSMKTPQIDELTSLSWIKQEDNPNKNLQSFFF
  IGQKLCEVTKDKITVFNYYPLEFSCANVTLYLYNLKTDDSGLYNGKAHTKELEHNTYVRLYVIDIPPPKC
  DITSRYLGIQATGEDYCLIEINCTNSKYPAVVKFNGRQSNFYHYVSENGNKKLPNFYETHITVNGTHKSF
  HFNYPFNDLCQTTSALQYNDNVQVVLILLIVVGLIIISASLILLYCHRKKIKAKVQHQPVHICLEK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 828 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040870
• RefSeq ORF: 828
• Locus ID: 2715934
• MW: 31.6 kDa