L1 (NC_001454) Virus Tagged ORF Clone

CAT#: VC100507

Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein 52K [Human adenovirus F], codon optimized for human cell expression, NP_040855

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100507 represents NCBI reference of NP_040855 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCCAGTGTTGAGGCAAATGAGACCCACCGCCCCCCCCACACAGCCCCCTCTGCCCCCTCCAACTT
  CAGCTCCGGCCGCTGTAGCTCTGAGTGGCGCTGGAGGAGGCAATCCAGAGGAAGAGGCCATTCTGGACCT
  GGAAGAGGGAGAGGGATTGGCACGGCTGGGTGCACCTAGTCCGGAGAGACACCCCAGGGTGCAGCTGAAG
  AAGGATAGTCGACAGGCATACGTTCCCCCACAGAATCTGTTTCGAGACAGATCAGGCCAGGAACCGGAGG
  AGATGAGGGACAGAAGGTTCTACGCTGGCCAAGAACTGCGGGCAGGCTTCAATCGACAGCGGGTGCTGCG
  GGCCGAAGACTTCGAGCCCGACGAGCATAGTGGCATATCCCCCGCCAGAGCACATGTCTCAGCAGCCGAT
  TTGGTCACGGCCTACGAACAGACAGTCAATGAAGAAAGAAATTTTCAGAAGTCTTTTAATAACCACGTGA
  GAACTCTGGTCGCAAGAGAGGAAGTTGCCATCGGCCTGATGCATCTGTGGGATTTTATGGAAGCATACGT
  GCAGAACCCCTCTAGTAAGCCGCTTACAGCCCAACTCTTTCTCATCGTCCAGCATAGCCGGGATAACGAA
  GCCTTCCGAGAGGCCATGCTGAACATAGCAGAGCCCGAAGGACGGTGGCTTCTCGATCTGGTGAATATTC
  TGCAGAGTATTGTCGTGCAGGAGAGAAGCCTGTCACTGGCTGACAAGGTGGCAGCTATCAATTATTCAAT
  GCTGAGCCTGGGCAAGTTTTACGCCCGCAAAATTTATAAAACTCCCTATGTTCCCATTGATAAAGAGGTG
  AAGATAGATTCATTTTACATGAGGATGGCCTTGAAGGTGCTGACACTTTCCGATGACCTGGGAGTGTATA
  GAAACGATCGGATACATAAAGCAGTTAGCGCTTCTCGCAGACGGGAGCTCAGTGACCGAGAACTTATGCA
  CAGCCTCAGGAGAGCACTGACTGGAACAGGAACTGACGCTGAGACAGAGTCTTACTTCGACATGGGCGCT
  GACCTGCAATGGCAACCTAGCGCTCGGGCACTGGAGGCAGCCGGGTATGTTGGGGCCGAGGAGGATGAAG
  AGGATTATGAGGATGAACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100507 representing NP_040855
  Red=Cloning sites Green=Tags
  
  MHPVLRQMRPTAPPTQPPLPPPTSAPAAVALSGAGGGNPEEEAILDLEEGEGLARLGAPSPERHPRVQLK
  KDSRQAYVPPQNLFRDRSGQEPEEMRDRRFYAGQELRAGFNRQRVLRAEDFEPDEHSGISPARAHVSAAD
  LVTAYEQTVNEERNFQKSFNNHVRTLVAREEVAIGLMHLWDFMEAYVQNPSSKPLTAQLFLIVQHSRDNE
  AFREAMLNIAEPEGRWLLDLVNILQSIVVQERSLSLADKVAAINYSMLSLGKFYARKIYKTPYVPIDKEV
  KIDSFYMRMALKVLTLSDDLGVYRNDRIHKAVSASRRRELSDRELMHSLRRALTGTGTDAETESYFDMGA
  DLQWQPSARALEAAGYVGAEEDEEDYEDEP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 1140 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040855
• RefSeq ORF: 1140
• Locus ID: 2715925
• MW: 42.9 kDa