L4A (NC_001454) Virus Tagged ORF Clone

CAT#: VC100517

Myc-DDK-tagged ORF clone of viral ORF for hexon assembly protein 100K [Human adenovirus F], codon optimized for human cell expression, NP_040865

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100517 represents NCBI reference of NP_040865 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAAGACTTGAAACTGCAGCCCGACTCAGAGACCCTGACAACCCCAAACTCCGAGGTGGGCGCCG
  TTGAATTGGTCAAACATGAGGAGGAAAACGAGCAGGTGGAGCAAGATCCCGGATATGTGACACCTCCCGA
  AGATGGTAAGGAACCCGTCGCCGCCCTCAGCGAGCCCAACTACTTGGGAGGCGAGGATGATGTTCTGCTC
  AAGCACATAGCGCGCCAATCCACCATAGTCCGCGAGGCACTGAAGGAGTGTACACAGACCCCGCTTACCG
  TGGAAGAGCTGAGTAGGGCCTACGAAGCAAACCTGTTTTCTCCAAGAGTACCACCCAAGAAGCAGCCGAA
  TGGAACGTGCGAAACCAACCCAAGGTTGAACTTTTATCCCGTGTTTGCCGTGCCTGAGGCTCTCGCCACC
  TACCACATTTTCTTCAAGAATCAGAGGATCCCACTCTCATGCCGCGCTAATCGGACCAGGGGGGATGGAC
  TGCTCCACCTGAAAGCTGGTGCGCACATCCCCGAAATAGTAAGTTTGGAGGAGGTCCCTAAGATTTTTGA
  GGGCCTGGGCAAGGACGAGAAGAGGGCAGCAAATGCCCTGCAGAAGAATGAAACTGAAAACCAGAATGTT
  CTCGTAGAGCTTGAGGGGGATAACGCACGCCTCGCGGTTCTGAAAAGGACAATTGAAGTGAGTCATTTTG
  CCTACCCCGCACTGAACCTCCCCCCAAAGGTTATGCGGTCTGTGATGGACCAGGTTCTGATCAAAAGAGC
  TGAGCCTATCGATCCACAACAACCAGACCTTAACTCCGAGGACGGGCAGCCCGTGGTCAGTGATGATGAG
  CTCGCTAGGTGGTTGGGCACTCAAGACCCATCCGAGTTGCAGGAAAGGCGAAAAATGATGATGGCGGCCG
  TTCTCGTGACTGTAGAGCTGGAGTGTCTGCAACGGTTCTTTGCCAACCCGCAAACCTTGCGCAAGGTCGA
  GGAGTCTCTGCATTATGCCTTTAGGCACGGGTACGTCCGACAGGCATGTAAAATTAGCAATGTGGAGCTT
  AGTAACCTGATTAGCTATATGGGCATACTTCATGAGAACAGGCTGGGACAGAACGTGCTGCACTGTACAC
  TTCAGGGGGAAGCCAGGCGGGATTATGTGAGAGACTGCATCTACTTGTTCTTGATACTGACCTGGCAGAC
  TGCAATGGGGGTCTGGCAACAATGTCTCGAGGAACAGAACCTGCAAGAGCTGAACAAACTGCTCGTTCGG
  GCACGCCGAGAGCTGTGGACTAGCTTCGACGAGCGAACGGTCGCCCGACAGCTGGCTAATCTCATTTTTC
  CAGAACGGCTGATGCAAACACTTCAGAACGGCCTGCCGGACTTTGTGTCACAGTCCATACTGCAAAACTT
  TCGGTCATTCGTCTTGGAAAGGTCCGGAATTCTTCCAGCTATGTCTTGCGCTCTGCCCAGCGACTTTGTC
  CCTCTTTGCTACCGCGAGTGCCCTCCTCCCCTTTGGAGCCACTGTTACCTGCTGAGGCTGGCTAATTACC
  TGGCGCATCATTCTGACCTTATGGAGGATTCCTCCGGGGACGGTCTGCTCGAGTGTCACTGCCGCTGCAA
  TCTGTGTACTCCCCACCGATCCCTGGTATGCAACACCGAACTGCTGAGCGAGACTCAGGTGATAGGGACT
  TTTGAGATTCAGGGACCCGAACAGCAGGAAGGAGCTAGCAGTCTGAAGCTGACACCAGCACTCTGGACCT
  CTGCCTACCTTAGGAAGTTTATTCCTGAGGACTATCACGCCCATCAGATTAAATTTTATGAAGACCAGTC
  CCGGCCTCCAAAAGTGCCATTGACCGCATGTGTAATCACACAATCCCAGATCCTTGCTCAGCTGCAGGCT
  ATCCAGCAGGCACGGCAGGAATTTCTGCTGAAAAAAGGGCATGGCGTCTACCTCGACCCTCAGACAGGCG
  AAGAGCTTAATACACCCTCTCTCAGTGCAGCAGCAAGCTGTCGAAGCCAGAAACACGCAACTCAGGGGAA
  GCAGGCTTCTCATAGAGCTACGGCCATACCTGCGGAGACTACCAAAGCTGTGGGGAGAGGAGGGGATGTC
  GGTCGCCAGCCCGGCCGGGGCTCCTTCAGACGAGGAGGCGGCGGTGCAGATGGTGAATTGGGGCAACCAA
  GAAGAGGCGGTCCAAGAGGTAGAGGCGGCAGAAATCACCGCCAGAGGCAGGGCACGATCTTTCAGAAGAC
  GAGGTCCGAGCCCACATCCGAGAATTACCCAGCACCAGCCACCGCAACAATGTTCACCGAGAGTCAACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100517 representing NP_040865
  Red=Cloning sites Green=Tags
  
  MEEDLKLQPDSETLTTPNSEVGAVELVKHEEENEQVEQDPGYVTPPEDGKEPVAALSEPNYLGGEDDVLL
  KHIARQSTIVREALKECTQTPLTVEELSRAYEANLFSPRVPPKKQPNGTCETNPRLNFYPVFAVPEALAT
  YHIFFKNQRIPLSCRANRTRGDGLLHLKAGAHIPEIVSLEEVPKIFEGLGKDEKRAANALQKNETENQNV
  LVELEGDNARLAVLKRTIEVSHFAYPALNLPPKVMRSVMDQVLIKRAEPIDPQQPDLNSEDGQPVVSDDE
  LARWLGTQDPSELQERRKMMMAAVLVTVELECLQRFFANPQTLRKVEESLHYAFRHGYVRQACKISNVEL
  SNLISYMGILHENRLGQNVLHCTLQGEARRDYVRDCIYLFLILTWQTAMGVWQQCLEEQNLQELNKLLVR
  ARRELWTSFDERTVARQLANLIFPERLMQTLQNGLPDFVSQSILQNFRSFVLERSGILPAMSCALPSDFV
  PLCYRECPPPLWSHCYLLRLANYLAHHSDLMEDSSGDGLLECHCRCNLCTPHRSLVCNTELLSETQVIGT
  FEIQGPEQQEGASSLKLTPALWTSAYLRKFIPEDYHAHQIKFYEDQSRPPKVPLTACVITQSQILAQLQA
  IQQARQEFLLKKGHGVYLDPQTGEELNTPSLSAAASCRSQKHATQGKQASHRATAIPAETTKAVGRGGDV
  GRQPGRGSFRRGGGGADGELGQPRRGGPRGRGGRNHRQRQGTIFQKTRSEPTSENYPAPATATMFTESQP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 2310 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040865
• RefSeq ORF: 2310
• Locus ID: 2715920
• MW: 86.5 kDa