E1B (NC_001454) Virus Tagged ORF Clone

CAT#: VC100502

Myc-DDK-tagged ORF clone of viral ORF for control protein E1B 55K [Human adenovirus F], codon optimized for human cell expression, NP_040850

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100502 represents NCBI reference of NP_040850 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACGGCCTAACAGCTCTGTGGCAGGTCTGTACAGCGGCTTGCACGGTAATGGATCAGTAGAGAACC
  TGGCGACTGAAGAGGAAGGTCTGAGGTTGCTGGCGGGAGCTGCTAGCGCACGGTTTGGCAGTTCAGCTGG
  TCGGGGCGGGGGTGGTGGGGAGCCTGAAGGAAGGCCTGGACCATTTAACGGGATCGTAACGGAACCCGAC
  CCCGAGGAGGGCACAAGTTCTGGACAGAGGGGCGGAATAAACGGACAGAGGGGCACCAAGAGAAAAATGG
  AGAACGAAGGGGAGGATTTCCTGAAGGAACTCACACTCAGCCTGATGAGCCGGCGGCATCATGAATCAGT
  GTGGTGGGCCGACCTGGAAGATGAGTTCAAGAATGGCGAGATGAATCTGCTCTATAAATATACTTTTGAA
  CAGCTGAAGACCCATTGGCTTGAGGCCTGGGAAGATTTCGAGCTGGCCCTTAATACATTTGCCAAGGTGG
  CGCTGAGGCCCGATACCATCTATACAATCAAGAAAACAGTGAATATCCGAAAGTGTGCCTACGTCCTGGG
  TAACGGAGCCGTGGTGCGATTCCAGACCTGCGACAGGGTGGCCTTTAACTGCGCAATGCAAAGTTTGGGA
  CCCGGTCTGATCGGCATGAGCGGCGTCACCTTTATGAACGTGAGATTCGTTGTGGAGGGGTTCAATGGCA
  CCGTATTTGCTTCTACAACCCAGCTGACGCTCCATGGCGTGTTCTTCCAGAACTGCTCCGGAATCTGTGT
  TGATAGTTGGGGAAGGGTCTCCGCCAGGGGCTGCACCTTTGTGGCATGTTGGAAAGGTGTGGTTGGACGA
  AACAAATCCCAAATGAGCGTAAAAAAATGTGTATTCGAGCGCTGCATCATGGCCATGGTGGTTGAGGGGC
  AAGCCCGAATTCGCCATAACGCAGGATCCGATAACGTGTGCTTCCTCCTCCTGAAGGGCACAGCGTCTGT
  AAAACACAACATGATTTGCGGCGGCGGACATTCTCAGCTGCTGACCTGCGCAGATGGGAACTGCCAGGCC
  CTGCGAGTCTTCCACGTGGTGAGCCATCCCAGGCGCCCTTGGCCCGTGTTCGAGCATAATATGCTGATGC
  GGTGTACCGTCCACTTGGGTGCCCGCCGAGGAATGTTCAGTCCGTACCAGTCAAACTTTTGTCACACAAA
  AGTCCTGATGGAGACCGATGCTTTCAGCCGAGTTTGGTGGAACGGGGTGTTCGACCTGACAATGGAACTG
  TTCAAAGTCGTTAGGTATGACGAATCAAAAGTAAGGTGTCGGCCCTGTGAGTGTGGTGCAAACCACATAC
  GCCTTTACCCTGCCACACTGAACGTGACTGAACAGCTGCGAACTGACCATCAGATGATGTCCTGTCTGAG
  GACCGACTATGAGTCTTCCGATGAAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100502 representing NP_040850
  Red=Cloning sites Green=Tags
  
  MERPNSSVAGLYSGLHGNGSVENLATEEEGLRLLAGAASARFGSSAGRGGGGGEPEGRPGPFNGIVTEPD
  PEEGTSSGQRGGINGQRGTKRKMENEGEDFLKELTLSLMSRRHHESVWWADLEDEFKNGEMNLLYKYTFE
  QLKTHWLEAWEDFELALNTFAKVALRPDTIYTIKKTVNIRKCAYVLGNGAVVRFQTCDRVAFNCAMQSLG
  PGLIGMSGVTFMNVRFVVEGFNGTVFASTTQLTLHGVFFQNCSGICVDSWGRVSARGCTFVACWKGVVGR
  NKSQMSVKKCVFERCIMAMVVEGQARIRHNAGSDNVCFLLLKGTASVKHNMICGGGHSQLLTCADGNCQA
  LRVFHVVSHPRRPWPVFEHNMLMRCTVHLGARRGMFSPYQSNFCHTKVLMETDAFSRVWWNGVFDLTMEL
  FKVVRYDESKVRCRPCECGANHIRLYPATLNVTEQLRTDHQMMSCLRTDYESSDED
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 1428 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040850
• RefSeq ORF: 1428
• Locus ID: 2715936
• MW: 53.0 kDa