L3 (NC_001454) Virus Tagged ORF Clone

CAT#: VC100515

Myc-DDK-tagged ORF clone of viral ORF for protease [Human adenovirus F], codon optimized for human cell expression, NP_040863

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100515 represents NCBI reference of NP_040863 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAGCTCCGAGCAAGAGCTGGTGGCAATCGTGAGAGAACTCGGCTGTGGACCTTACTTCCTCGGTA
  CTTTCGACAAACGGTTCCCAGGCTTCATGGCTCCGCACAAGCTGGCCTGCGCAATCGTTAATACAGCCGG
  CCGCGAAACCGGAGGAGTCCACTGGCTGGCCCTTGCTTGGAACCCTAAGAACCGAACTTGCTACCTGTTC
  GACCCGTTTGGTTTTAGTGACGAACGACTGAAGCAGATATATCAATTCGAATACGAGGGGCTGCTGAAGC
  GCTCCGCCCTGGCCTCTACGCCTGACCACTGTATCACATTGATCAAGTCCACACAGACTGTGCAGGGGCC
  TTTTTCAGCTGCGTGCGGCCTCTTTTGTTGTATGTTCCTGCATGCATTTGTGAATTGGCCCACATCTCCA
  ATGGAGCGAAACCCAACGATGGACCTGCTGACCGGCGTGCCAAATTCAATGCTCCAGAGTCCACAGGTGG
  TGCCAACCCTGCGGCACAACCAGGAGAGACTGTACCGCTTTCTCGCACAGCGGAGCCCATATTTTCAGCG
  CCACTGCGAGCGGATTAAGAAGGCTACCGCCTTTGATCAGATGAAAAACAACATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100515 representing NP_040863
  Red=Cloning sites Green=Tags
  
  MGSSEQELVAIVRELGCGPYFLGTFDKRFPGFMAPHKLACAIVNTAGRETGGVHWLALAWNPKNRTCYLF
  DPFGFSDERLKQIYQFEYEGLLKRSALASTPDHCITLIKSTQTVQGPFSAACGLFCCMFLHAFVNWPTSP
  MERNPTMDLLTGVPNSMLQSPQVVPTLRHNQERLYRFLAQRSPYFQRHCERIKKATAFDQMKNNM
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 615 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040863
• RefSeq ORF: 615
• Locus ID: 2715932
• MW: 23.3 kDa