E2B (NC_001454) Virus Tagged ORF Clone

CAT#: VC100506

Myc-DDK-tagged ORF clone of viral ORF for terminal protein precursor pTP [Human adenovirus F], codon optimized for human cell expression, NP_040854

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100506 represents NCBI reference of NP_040854 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATTGAGCGTGCAAGACTGCGCACGGCTCACCGGGCAGTCAGTTCCAGCCATGGAACGGTTCAGTC
  CATTGCGGAACATCTGGAACCGGGTTAGGGAGTATGCTAGGGCAGCAACCACGGCAGCCGGCATCACATG
  GCTGAGCCGCTATGTCTACCACTATCATCGCTTGATGCTCGAGGACCTGGCGCCGGGCACACCAGCGACA
  CTTAGGTGGCCATTGTATCGCGAGCCCCCTCCTCACTTTTTGGTGGGCTATCAGTACCTGGTTCGCACCT
  GCAACGATTATGTTTTCGAGTCTAGGGCCTACAGTCGGCTGCGCTACACTGAGATTACACAGCCCGGCAT
  GCAAGTGGTTAATTGGAGTGTCATGGCGAATTGTACCTACACTATCAATACCGGGAGCTACCATAGGTTC
  GTAGATCTTGATGACTTCCAGAACACATTGACCCAGATTCAACAGGCAGTGCTGGCCGAAAGAGTGGTCG
  CCGATTTGGCACTGCTGCAGCCCCTCCGGGGTTTCGGAAGTACAAGAATGGCTGATAGGGGAGAACTGGA
  AATTCCTGTCGAGTCTCTCATGCAGGACTATTATAAGGATCTGCGCAGGTGTCAAAATGAAGCATGGGGG
  ATGGCAGACCGACTCCGCATCCAACAGGCAGGTCCAAAAGATGTGACCCTGCTGGCAACAATTCGACGCC
  TCAAGACCGCATACTTCAACTTTCTGATCTCATCTATTACCTCCTCCGCGGCATCACTGCGAATCCCTCA
  CGCCACAGTCCTTAGTCTCCCATGCGACTGCGACTGGCTCGAAGCCTTCCTGGAAAAGTTCAGCGATCCG
  GTCCAGCTTGACTCCCTGAATGAAGCCTGGCAGTCTCTCCCGATGCAGCAGATGATTAGGTGCACGGTGT
  CAGCCCTGTCACTGCCCCAGGGGCCTCACTTGCTGCCGCCTCTTTCCGGGAGTGGAATGCAGGGGGGTGT
  ATTCGAGCTCCGGCCACGAGAGAACGGTCGGGCCGTGACTGAGACAATGCGCAGAAGGAGAGGTGAAATG
  ATACAACGCTTTATTGACAGGCTGCCTGTCCGCAGAAGGAGGCGGAGACAGCCGGTGCCCGCCCCTGTGA
  GCCCTGAAGGACCCGCCGTTGAGGAAGAGGAATTTATGGAGATTGAGGAGACTCCAGCGGCGTTCGAGCA
  GGAAGTCAGAGAAACCGTGGCCGAAGCAATTAGACTGCTTCAGGAGGAGCTGACCTTCGCAGCTAGGAAC
  TCCCAATTTTTTAACTTCGCAGTGGATTTTTACGAGGCTATGGATAGACTGGAAGCGCTGGGCGATATAA
  ACGAAATGACACTCAGACGATGGGTGATGTATTTTTTTGTGTGCGAACACATCGCTACAACTCTCAACTA
  TCTCTTCCAGCGCCTCCGAAATTATGCCGTCTTCGCGAGACATGTAGAACTGAATATTGCGCAGGTGGTC
  ATGCGAGCCCGCAATACCGTCGGTGATGTCGTATATTCCCGCGTTTGGAACGAGAACGGACTCAACGCAT
  TTAGTCAACTCATGAGGCGCATCAGTAATGATCTCGCTGCTACCGTTGAAAGGGCAGGCCATGGCGAACT
  GCAGGACGAGGAGATCGACCAATTTATGTCAGAAATCGCCTATCAGGATAATAGCGGGGATGTGCAGGAA
  ATACTCAGGCAGGCTGCAGTCAATGACGCAGATATCGACTCAGTCGAGCTTTCTTTTAGGTTTAGAGTCC
  GCGGGCCTGTTGTGTTCAGTCAGCGCCGGCATATCCAGGACCTCAACCGGCGGGTGGTCGCATACGCATC
  CCAGCTCCGCGCTCAACACCAGCCGCTGCCTGAACTTCACGCCGATGTCCCATTGCCTCCTCTCCAAGCC
  AATCCACACCCACCACTTCCTCCCGATGCTAGACCACAGCGGACCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100506 representing NP_040854
  Red=Cloning sites Green=Tags
  
  MALSVQDCARLTGQSVPAMERFSPLRNIWNRVREYARAATTAAGITWLSRYVYHYHRLMLEDLAPGTPAT
  LRWPLYREPPPHFLVGYQYLVRTCNDYVFESRAYSRLRYTEITQPGMQVVNWSVMANCTYTINTGSYHRF
  VDLDDFQNTLTQIQQAVLAERVVADLALLQPLRGFGSTRMADRGELEIPVESLMQDYYKDLRRCQNEAWG
  MADRLRIQQAGPKDVTLLATIRRLKTAYFNFLISSITSSAASLRIPHATVLSLPCDCDWLEAFLEKFSDP
  VQLDSLNEAWQSLPMQQMIRCTVSALSLPQGPHLLPPLSGSGMQGGVFELRPRENGRAVTETMRRRRGEM
  IQRFIDRLPVRRRRRRQPVPAPVSPEGPAVEEEEFMEIEETPAAFEQEVRETVAEAIRLLQEELTFAARN
  SQFFNFAVDFYEAMDRLEALGDINEMTLRRWVMYFFVCEHIATTLNYLFQRLRNYAVFARHVELNIAQVV
  MRARNTVGDVVYSRVWNENGLNAFSQLMRRISNDLAATVERAGHGELQDEEIDQFMSEIAYQDNSGDVQE
  ILRQAAVNDADIDSVELSFRFRVRGPVVFSQRRHIQDLNRRVVAYASQLRAQHQPLPELHADVPLPPLQA
  NPHPPLPPDARPQRTM
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 1938 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040854
• RefSeq ORF: 1938
• Locus ID: 2715933
• MW: 74.3 kDa