E4 (NC_001454) Virus Tagged ORF Clone

CAT#: VC100530

Myc-DDK-tagged ORF clone of viral ORF for control protein E4 34K [Human adenovirus F], codon optimized for human cell expression, NP_040878

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100530 represents NCBI reference of NP_040878 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCAACGGGATCGATGGTTCAGATGTCGGCTGAGTAGCTACCAGACGCACAGACTGCCTCCCCCTT
  CCGAAGACTCTGTGCCGATCGCTACAATGGAGCGGACTCCCGGCCTCTTGGAATGCGAGCATCTCAATAT
  GCATTACGTGTCCGAAGTCAGGAGCATTCCCTCTTGCGCACCCTTCGTGGTGTTGCAGGAGTGGCCCATG
  TGGTGGGATATGATCTTGTCAAAGTGGGAAAAGCATGTCTGCAAGATTTATATGCGCGTTTGCGTCTGCT
  GTGCCACTATTAACGTCGACTTCAACCAGATTGTTAGAGGTTTCGAACGGTGGACCATCCATTGCCACTG
  CATTAAGCCCGGCAGCCTGCAATGCAAGGCTGGCGGGGTGGTTCTGGCTAATTGGTTTAAAATGATGATT
  TACGGGTCTCTCTATAATTTGAGGTTTCCATGGTATCGCGAAATCGTGAACTGTGGGATGCCAAAAGAAG
  TTCTGTTTATGGGCTCTGTGTTTGTCCGAGGACGGCACTTCATTTATATCCGCCTCTTGTATGATGGTCA
  TGCTGCGATCGTGCTGGAGAGGATGTCTTTCGGCTGGTCCTTCTTTAGCTATGGAATCATGAATAACATG
  GTCGTACTGGGATGTACTTACTGTAAAAATCTGGACGAGCTCTCAGTGCGGTGCTGCGCCCGGAGAACAA
  GACGCCTGCTGGCAAAGGCAGTCAAAGTGCTGGGTTCCTTTACCGTGAGAAGTCTTATGAGATCTCTGGT
  GGAACCCCGGCGGCAGGGCCTTCTGCGAGGTCTCATGGAGCGGTACTGTCCATTTACACTGGCCGACTAT
  AACCGGGGAGAAAACCCCTGGCGCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100530 representing NP_040878
  Red=Cloning sites Green=Tags
  
  MMQRDRWFRCRLSSYQTHRLPPPSEDSVPIATMERTPGLLECEHLNMHYVSEVRSIPSCAPFVVLQEWPM
  WWDMILSKWEKHVCKIYMRVCVCCATINVDFNQIVRGFERWTIHCHCIKPGSLQCKAGGVVLANWFKMMI
  YGSLYNLRFPWYREIVNCGMPKEVLFMGSVFVRGRHFIYIRLLYDGHAAIVLERMSFGWSFFSYGIMNNM
  VVLGCTYCKNLDELSVRCCARRTRRLLAKAVKVLGSFTVRSLMRSLVEPRRQGLLRGLMERYCPFTLADY
  NRGENPWRA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 867 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040878
• RefSeq ORF: 867
• Locus ID: 2715935
• MW: 34.0 kDa