E4 (NC_001454) Virus Tagged ORF Clone

CAT#: VC100533

Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf2 [Human adenovirus F], codon optimized for human cell expression, NP_040881

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100533 represents NCBI reference of NP_040881 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATCTGTTGAGGCCCGTGTTTATTTCCGTTGTGGTTCCCGAACCTCTGATGTTTTATCTGAATAACA
  TGGATATATCCGTCGTAGATTTCCTCAAAACAAATATGTCAGAATTTCTGCTGCACATTCTGCATTGCGT
  GACCCCCGTGCTTCAGGCAGCTTACTGTGGTTTCACACTCACATCCCTCGCCCCCACACTGGAGATGATT
  TGTTGCGTCGCAAGCCCGGAACTTCTCCCAGACTCAGAGCTTACAGAGCTCATCTGTAACGATCTGGACG
  ACTTCGTACAACTCATGCTCAGGATGGAAATCCGGGACAGAGGGTTCGAGCCTAATGTGAACCTGTTGAA
  CGAGCTTCAGGTCACCAGAGAGCCTGATCTTTTTCAGCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100533 representing NP_040881
  Red=Cloning sites Green=Tags
  
  MYLLRPVFISVVVPEPLMFYLNNMDISVVDFLKTNMSEFLLHILHCVTPVLQAAYCGFTLTSLAPTLEMI
  CCVASPELLPDSELTELICNDLDDFVQLMLRMEIRDRGFEPNVNLLNELQVTREPDLFQP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 390 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040881
• RefSeq ORF: 390
• Locus ID: 2715935
• MW: 14.9 kDa