IVa2 (NC_001454) Virus Tagged ORF Clone

CAT#: VC100504

Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein IVa2 [Human adenovirus F], codon optimized for human cell expression, NP_040852

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IVa2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100504 represents NCBI reference of NP_040852 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCAGGGGTCGAAGAAGGGCATTCAGCCACCAGCAGGACGAGCCAGAGGAAAACCCCGGTAAAC
  GGCCCACGCGGTCAGCTCCTTTGTACCGGCATCGCAATCAGCCTAATGCAAATCCCGCGACTCTTGAACG
  CCATTATCCCTGTTCTACCGGTCGACCCCCTACCGGCACCGTGCAGCCTAAGCCTAGCCAGCCGCCTCAA
  CCCCGCTCTCTGCTGGATCGGGACGCAATTGATCACATCACTGAACTTTGGGATCGCTTGTATCTGCTGC
  GCCAGTCACTGGAGAAAATGACTATGGCTGACGGTTTGAAGCCACTCAAGCACTTTCGGTCACTGGAGGA
  ACTCCTCTCTTTGGGCGGAGAAAGGCTCCTGCAGGATTTGGTGAAGGAGAACCAGCACGTTAGAAGCATG
  ATGAATGAGGTGACCCCACTCCTGCGGGAGGACGGATCCTGTATTTCACTGAACTATCAGCTGCAACCGG
  TGATTGGCGTGATATACGGCCCTACAGGCTGCGGGAAGTCTCAGTTGCTGCGAAATCTTTTGTCTACCCA
  ACTGATTAATCCACCACCTGAGACAGTGTTTTTCATTGCACCCCAGGTTGATATGATACCCCCTTCCGAG
  ATTAAAGCCTGGGAGATGCAAATCTGCGAAGGCAATTACGCTCCAGGACCGGAGGGCACGATAATCCCTC
  AAAGCGGGACTCTGCTGCCTCGGTTCATAAAGATGGCGTATGACGAACTGACGCTCGAACAGAATTACGA
  CGTGAGTCACCCGGACAATATTTTTGCCAAGGCGGCAAGTCAAGGTCCTATCGCCATCATCATGGATGAA
  TGCATGGAGAACCTGGGCGGACATAAGGGAGTTTCCAAGTTCTTCCATGCTTTTCCAAGCAAGCTGCACG
  ACAAGTTTCCCAAGTGTACCGGTTACACAGTACTCGTTGTACTGCACAACATGAATCCGAGACGAGACCT
  CGGCGGAAACATTGCAAATCTGAAGATTCAGGCTAAGATGCACCTGATCTCACCGAGAATGCATCCTTCC
  CAACTCAACAGATTCGTGAATACATTCACCAAAGGACTCCCACTTGCTATTTCCCTGCTGCTTAAGGACA
  TTTTTCAATTCCACGCTCAGAAACCGTGCTACGACTGGATCATATATAATACCACGCCTGAGCACGACGC
  ACTGCAATGGTCATACCTCCACCCGCGGGATGGGCTTATGCCCATGTATTTGAATATACAGGCTCACCTG
  TATCGGGTCTTGGAAAATATCCACAAAGTTCTGAATGACCGGGACAGATGGAGCAGAGCCTACAGAAAGC
  GCAATAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100504 representing NP_040852
  Red=Cloning sites Green=Tags
  
  METRGRRRAFSHQQDEPEENPGKRPTRSAPLYRHRNQPNANPATLERHYPCSTGRPPTGTVQPKPSQPPQ
  PRSLLDRDAIDHITELWDRLYLLRQSLEKMTMADGLKPLKHFRSLEELLSLGGERLLQDLVKENQHVRSM
  MNEVTPLLREDGSCISLNYQLQPVIGVIYGPTGCGKSQLLRNLLSTQLINPPPETVFFIAPQVDMIPPSE
  IKAWEMQICEGNYAPGPEGTIIPQSGTLLPRFIKMAYDELTLEQNYDVSHPDNIFAKAASQGPIAIIMDE
  CMENLGGHKGVSKFFHAFPSKLHDKFPKCTGYTVLVVLHNMNPRRDLGGNIANLKIQAKMHLISPRMHPS
  QLNRFVNTFTKGLPLAISLLLKDIFQFHAQKPCYDWIIYNTTPEHDALQWSYLHPRDGLMPMYLNIQAHL
  YRVLENIHKVLNDRDRWSRAYRKRNK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 1338 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040852
• RefSeq ORF: 1338
• Locus ID: 2715937
• MW: 51.1 kDa