L2 (NC_001454) Virus Tagged ORF Clone

CAT#: VC100509

Myc-DDK-tagged ORF clone of viral ORF for penton base [Human adenovirus F], codon optimized for human cell expression, NP_040857

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100509 represents NCBI reference of NP_040857 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCGGGCAGTTGGCGTGCCTCCAGTCATGGCCTACGCTGAAGGACCTCCACCAAGCTACGAATCTG
  TTATGGAGACCGCAGATCTCCCCGCTACCTTGCAAGCCCTGCATGTCCCCCCTCGGTATCTCGGGCCTAC
  CGAAGGGAGAAACTCTATTCGATATTCCGAACTTGCCCCCCTCTATGATACTACACGGGTCTACTTGGTG
  GACAACAAATCCGCCGATATTGCATCCCTGAATTACCAGAATGACCATTCCAATTTCCAAACTACAGTTG
  TTCAGAACAATGATTTTACTCCAACCGAGGCCGGTACCCAGACCATCAACTTCGATGACCGCTCTCGGTG
  GGGGGGCGACCTGAAAACAATTCTGAGGACAAACATGCCGAACATTAATGAATTTATGAGCACCAACAAG
  TTTAGAGCCCGGGTGATGGTTGAGAAGGTCAATCGCAAGACTAACGCACCAAGGTACGAATGGTTCGAAT
  TTACACTCCCCGAGGGCAACTATAGCGAAACTATGACCATCGACCTGATGAACAACGCAATCGTGGATAA
  TTATCTTGCTGTGGGAAGACAGAATGGGGTTCTGGAATCAGATATTGGCGTGAAATTTGACACCAGGAAT
  TTTCGACTCGGTTGGGATCCCGTTACTAAGCTGGTGATGCCAGGCGTGTACACAAATGAGGCTTTTCATC
  CCGATATTGTACTTCTGCCAGGCTGCGGTGTAGATTTCACCCAGAGCCGGCTGAACAACCTGCTGGGCAT
  TAGAAAGAGGATGCCTTTTCAGAAAGGTTTCCAAATCATGTATGAGGATTTGGAGGGCGGAAACATCCCA
  GCCCTGCTGGACGTTGAGAAATACGAAGCGAGTATTAAGGAAGCTCAGGAAATACGCGGCGCTGACTTCA
  AGCCAAACCCCCAGGACCTCGAGATTGTGCCCGTTGAGAAAGATAGCAAGGAGAGGTCATACAATTTGCT
  GGAAGGGGACAAGAACAATACTGCATACCGCAGTTGGTTCCTGGCTTATAATTACGGTGATGCCGAAAAG
  GGCGTCAAGTCTTGGACTCTGCTGACGACAACAGATGTGACATGTGGATCTCAGCAAGTGTATTGGTCCC
  TGCCCGATATGATGCAAGACCCTGTCACATTCAGGCCTTCAACCCAAGTGAGCAATTACCCTGTTGTCGG
  CGTGGAACTCCTGCCAGTTCATGCAAAATCCTTCTACAATGAGCAGGCCGTTTATTCCCAGCTCATCAGG
  CAGTCTACGGCTCTCACACATATCTTTAATAGGTTCCCTGAAAACCAGATTCTGGTTCGGCCACCAGCAC
  CCACCATTACCACCGTCTCCGAAAATGTGCCAGCTCTGACCGACCATGGTACACTTCCACTGCGCTCTTC
  CATTAGCGGGGTGCAGAGAGTCACGATTACTGACGCTCGGCGAAGGACTTGTCCCTACGTACACAAGGCC
  CTGGGCATTGTCGCCCCAAAAGTGCTCAGTTCCCGAACCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100509 representing NP_040857
  Red=Cloning sites Green=Tags
  
  MRRAVGVPPVMAYAEGPPPSYESVMETADLPATLQALHVPPRYLGPTEGRNSIRYSELAPLYDTTRVYLV
  DNKSADIASLNYQNDHSNFQTTVVQNNDFTPTEAGTQTINFDDRSRWGGDLKTILRTNMPNINEFMSTNK
  FRARVMVEKVNRKTNAPRYEWFEFTLPEGNYSETMTIDLMNNAIVDNYLAVGRQNGVLESDIGVKFDTRN
  FRLGWDPVTKLVMPGVYTNEAFHPDIVLLPGCGVDFTQSRLNNLLGIRKRMPFQKGFQIMYEDLEGGNIP
  ALLDVEKYEASIKEAQEIRGADFKPNPQDLEIVPVEKDSKERSYNLLEGDKNNTAYRSWFLAYNYGDAEK
  GVKSWTLLTTTDVTCGSQQVYWSLPDMMQDPVTFRPSTQVSNYPVVGVELLPVHAKSFYNEQAVYSQLIR
  QSTALTHIFNRFPENQILVRPPAPTITTVSENVPALTDHGTLPLRSSISGVQRVTITDARRRTCPYVHKA
  LGIVAPKVLSSRTF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 1512 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040857
• RefSeq ORF: 1512
• Locus ID: 2715931
• MW: 56.9 kDa