E3B (NC_001454) Virus Tagged ORF Clone

CAT#: VC100523

Myc-DDK-tagged ORF clone of viral ORF for membrane protein E3 RID-alpha [Human adenovirus F], codon optimized for human cell expression, NP_040871

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100523 represents NCBI reference of NP_040871 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCACCCCCCTCCTCCTCCTTGTATGTTTGCCAATTATATATGCCTCCACAACTTTCGCCGCTGTAT
  CCCATCTCGATACAGATTGTTTGCCAGCCCTCTTGACTTATCTGATCTTTACTTCAGTGTGTTGTACCGC
  TATTTGCAGCATTGCAACCTTCTTTGTGGCCATCTTTCAGACTGCCGACTATCTCTACGTAAGAGTTGCC
  TACTACCGACACCACCCTCAGTACCGCAACCACGAGGTCGCCACTCTTTTGTGTCTGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100523 representing NP_040871
  Red=Cloning sites Green=Tags
  
  MVTPLLLLVCLPIIYASTTFAAVSHLDTDCLPALLTYLIFTSVCCTAICSIATFFVAIFQTADYLYVRVA
  YYRHHPQYRNHEVATLLCLS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 270 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040871
• RefSeq ORF: 270
• Locus ID: 2715919
• MW: 10.1 kDa