L3 (NC_001454) Virus Tagged ORF Clone

CAT#: VC100513

Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pVI [Human adenovirus F], codon optimized for human cell expression, NP_040861

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100513 represents NCBI reference of NP_040861 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGATATCAATTTCGCTTCTCTTGCCCCCAGGCATGGATCAAGACCTTTCATGGGGACCTGGAACG
  AAATAGGAACCTCTCAACTTAACGGCGGAGCTTTCTCATGGTCAAGCTTGTGGTCAGGCATTAAAAATTT
  TGGCAGCTCTATCAAGTCATTTGGCAACAAAGCATGGAATTCCAATACCGGTCAAATGCTGAGAGATAAA
  CTTAAGGATCAAAACTTTCAGCAGAAGGTGGTGGACGGATTGGCATCAGGCATAAATGGCGTAGTGGACA
  TTGCCAATCAGGCACTCCAAAATCAGATAAATCAGCGGCTGGAGAACTCAAGGCAACCGCCGGTGGCATT
  GCAGCAGCGGCCGCCACCCAAAGTTGAAGAGGTGGAAGTAGAGGAGAAACTCCCTCCCTTGGAGGTGGCG
  CCTCCCCTCCCGAGTAAGGGCGAAAAAAGGCCGAGGCCCGATTTGGAAGAAACACTTGTGGTGGAGAGTC
  GGGAACCCCCGAGTTACGAACAGGCCCTGAAGGAGGGGGCCTCACCCTACCCCATGACTAAGCCCATAGG
  GAGCATGGCCCGGCCCGTGTATGGCAAAGAGTCTAAACCTGTGACACTGGAGCTGCCCCCTCCTGTTCCC
  ACCGTTCCTCCCATGCCCGCACCAACACTCGGCACTGCTGTGTCACGCCCAACCGCTCCCACAGTCGCCG
  TGGCAACGCCTGCCAGAAGGCCTAGAGGCGCCAATTGGCAAAGCACACTGAATTCAATTGTGGGCCTGGG
  TGTGAAGTCCCTTAAGAGGAGGCGGTGCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100513 representing NP_040861
  Red=Cloning sites Green=Tags
  
  MEDINFASLAPRHGSRPFMGTWNEIGTSQLNGGAFSWSSLWSGIKNFGSSIKSFGNKAWNSNTGQMLRDK
  LKDQNFQQKVVDGLASGINGVVDIANQALQNQINQRLENSRQPPVALQQRPPPKVEEVEVEEKLPPLEVA
  PPLPSKGEKRPRPDLEETLVVESREPPSYEQALKEGASPYPMTKPIGSMARPVYGKESKPVTLELPPPVP
  TVPPMPAPTLGTAVSRPTAPTVAVATPARRPRGANWQSTLNSIVGLGVKSLKRRRCY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 801 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040861
• RefSeq ORF: 801
• Locus ID: 2715932
• MW: 29.1 kDa