L5A (NC_001454) Virus Tagged ORF Clone

CAT#: VC100528

Myc-DDK-tagged ORF clone of viral ORF for fiber-2 [Human adenovirus F], codon optimized for human cell expression, NP_040876

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L5A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100528 represents NCBI reference of NP_040876 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGCGCTAGGTTTGAGGACGACTTTAATCCGGTATACCCATACGAACACTACAACCCCCTCGATA
  TACCTTTCATCACCCCTCCCTTCGCCAGTTCTAACGGCCTCCAAGAAAAGCCCCCCGGTGTTTTGAGTCT
  GAAGTACACCGACCCACTTACAACCAAGAATGGAGCGCTCACGTTGAAACTCGGAACTGGTCTTAATATC
  GATAAAAACGGCGATCTTTCATCCGATGCGAGCGTCGAAGTGTCCGCTCCCATTACCAAGACCAACAAAA
  TCGTGGGCCTTAATTACACTAAGCCACTCGCACTTCAGAACAACGCACTGACATTGTCTTATAACGCGCC
  ATTCAATGTGGTCAATAATAACCTCGCACTCAATATGTCCCAGCCCGTGACAATCAATGCCAATAACGAA
  TTGAGTTTGCTGATCGACGCTCCACTGAATGCAGATACCGGAACCTTGCGGCTGAGAAGTGACGCCCCAC
  TCGGCCTCGTAGACAAGACTTTGAAAGTGCTCTTCTCTTCCCCGCTCTACCTGGATAACAACTTTTTGAC
  CCTCGCCATCGAAAGGCCGCTCGCCCTGAGCTCCAACAGAGCTGTGGCCCTTAAATACTCTCCACCGTTG
  AAGATCGAAAACGAGAATCTGACTCTGTCCACAGGGGGACCATTTACTGTTTCCGGGGGAAATCTGAACC
  TGGCCACATCTGCACCTTTGAGCGTGCAGAATAATTCTCTTTCACTCGGGGTAAATCCACCCTTTCTGAT
  TACTGACTCAGGACTTGCCATGGACCTTGGGGACGGGCTGGCCCTCGGCGGCAGCAAGCTGATTATTAAT
  CTGGGACCAGGACTGCAGATGTCCAACGGCGCCATCACTTTGGCCCTTGATGCTGCGCTTCCACTGCAAT
  ACAAAAATAATCAGCTTCAGCTCAGAATAGGCTCTGCTTCTGCATTGATTATGTCCGGGGTGACCCAGAC
  ACTGAACGTGAATGCAAACACAAGTAAAGGGTTGGCTATCGAAAACAACAGCCTCGTTGTCAAGCTTGGT
  AATGGGCTCAGATTTGATTCATGGGGTTCCATCGCTGTGTCACCAACCACCACTACTCCTACAACACTGT
  GGACCACCGCTGATCCTAGTCCCAATGCCACTTTTTACGAGTCCCTGGACGCCAAAGTGTGGCTGGTGCT
  CGTCAAGTGCAATGGCATGGTGAACGGAACGATCTCCATCAAGGCTCAAAAGGGTACCTTGTTGAAACCA
  ACTGCTAGCTTTATTTCATTTGTGATGTATTTCTACAGTGACGGGACCTGGAGGAAAAATTACCCTGTAT
  TCGACAATGAGGGAATCCTGGCGAATTCAGCAACCTGGGGATATAGACAGGGGCAGTCCGCAAACACTAA
  CGTGAGCAACGCGGTCGAATTCATGCCCAGCAGCAAGCGGTACCCTAACGAAAAAGGCTCTGAGGTCCAA
  AACATGGCTCTTACATACACATTCCTGCAGGGCGACCCCAACATGGCCATATCTTTCCAAAGCATCTACA
  ACCACGCAATTGAAGGTTACAGTCTGAAGTTTACCTGGAGGGTCCGCAACAATGAGCGGTTTGATATCCC
  ATGCTGTAGTTTTTCATATGTGACAGAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100528 representing NP_040876
  Red=Cloning sites Green=Tags
  
  MKRARFEDDFNPVYPYEHYNPLDIPFITPPFASSNGLQEKPPGVLSLKYTDPLTTKNGALTLKLGTGLNI
  DKNGDLSSDASVEVSAPITKTNKIVGLNYTKPLALQNNALTLSYNAPFNVVNNNLALNMSQPVTINANNE
  LSLLIDAPLNADTGTLRLRSDAPLGLVDKTLKVLFSSPLYLDNNFLTLAIERPLALSSNRAVALKYSPPL
  KIENENLTLSTGGPFTVSGGNLNLATSAPLSVQNNSLSLGVNPPFLITDSGLAMDLGDGLALGGSKLIIN
  LGPGLQMSNGAITLALDAALPLQYKNNQLQLRIGSASALIMSGVTQTLNVNANTSKGLAIENNSLVVKLG
  NGLRFDSWGSIAVSPTTTTPTTLWTTADPSPNATFYESLDAKVWLVLVKCNGMVNGTISIKAQKGTLLKP
  TASFISFVMYFYSDGTWRKNYPVFDNEGILANSATWGYRQGQSANTNVSNAVEFMPSSKRYPNEKGSEVQ
  NMALTYTFLQGDPNMAISFQSIYNHAIEGYSLKFTWRVRNNERFDIPCCSFSYVTEQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 1641 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040876
• RefSeq ORF: 1641
• Locus ID: 2715916
• MW: 59.1 kDa