E1B (NC_001454) Virus Tagged ORF Clone

CAT#: VC100501

Myc-DDK-tagged ORF clone of viral ORF for control protein E1B 19K [Human adenovirus F], codon optimized for human cell expression, NP_040848

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100501 represents NCBI reference of NP_040848 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCTGGAGTGAGTTGCAGAGCTACCAGAATCTGCGACGACTGCTGGAGCTCGCCTCCGCCCGCA
  CCTCCTCCTGTTGGAGAATCCTTTTCGGCTCCACCCTCACCAATGTGATCTACCGGGCCAAAGAAGAGTA
  TTCATCCCGCTTCGCAGACCTGCTCTCACACAACCCTGGGATTTTTGCATCACTGAACCTGGGGCACCAT
  TCCTTTTTCCAGGAAATCGTTATACGGAACCTGGATTTTTCCTCACCTGGGAGAACAGTGAGTGGACTGG
  CCTTTATATGTTTCATCCTTGACCAATGGTCAGCACAGACACACCTGAGTCAGGGCTACACCCTGGACTA
  CATGGCTATGGCCCTGTGGCGCACGCTGCTTAGGCGGAAACGGGTGCTGGGGTGCCTTCCTGCGCAGCGG
  CCCCACGGGCTCGATCCCGTGCAGGAGGAAGAAGAGGAAGAAGAAAACCTTAGGGCAGGCCTGGACCCCT
  CCACCGAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100501 representing NP_040848
  Red=Cloning sites Green=Tags
  
  MELWSELQSYQNLRRLLELASARTSSCWRILFGSTLTNVIYRAKEEYSSRFADLLSHNPGIFASLNLGHH
  SFFQEIVIRNLDFSSPGRTVSGLAFICFILDQWSAQTHLSQGYTLDYMAMALWRTLLRRKRVLGCLPAQR
  PHGLDPVQEEEEEEENLRAGLDPSTEL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 501 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040848
• RefSeq ORF: 501
• Locus ID: 2715936
• MW: 19.2 kDa