E3B (NC_001454) Virus Tagged ORF Clone

CAT#: VC100525

Myc-DDK-tagged ORF clone of viral ORF for control protein E3 147K [Human adenovirus F], codon optimized for human cell expression, NP_040873

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100525 represents NCBI reference of NP_040873 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGACCAGTTGGAGATCGACGGACAATGCACCGAACAGCTTATCCTGGCCCGGCGGAAGCTGAAGC
  AGCAGAACCAGGAATTGTTCAACCTGCAGGCTCTCCACCAGTGCAAAAAGGGTTTGTTCTGCCTTGTGAA
  GCAGGCCGAGTTGTGTTATGATGTGACCCAGCAGGGACATGAACTCTCCTACACTCTCAATAAACAGCGC
  CAGAGTTTCATGACCATGGTAGGCGTAAAACCAATCAAGGTGACTCAGCAGAGCGGGCCAGTGGAGGGCT
  CTATCCTGTGCCAGTGCACCAATCCAGAATGCATGTATACAATGGTCAAAACACTGTGTGGACTGAGGGA
  GCTGCTCCCATTTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100525 representing NP_040873
  Red=Cloning sites Green=Tags
  
  MSDQLEIDGQCTEQLILARRKLKQQNQELFNLQALHQCKKGLFCLVKQAELCYDVTQQGHELSYTLNKQR
  QSFMTMVGVKPIKVTQQSGPVEGSILCQCTNPECMYTMVKTLCGLRELLPFN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 366 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040873
• RefSeq ORF: 366
• Locus ID: 2715919
• MW: 13.9 kDa