L4A (NC_001454) Virus Tagged ORF Clone

CAT#: VC100519

Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein 22K [Human adenovirus F], codon optimized for human cell expression, NP_040867

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L4A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100519 represents NCBI reference of NP_040867 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCCGAAAGGCAATAAGCACCCTATAGCTCAGCGGCAGTCCCAGCAAAAGCTCCAAAAACAATGGG
  ATGAGGAAGAGACATGGGACGATAGCCAAGCGGAGGAAGTTAGTGACGAAGAAGCTGAGGAGCAAATGGA
  GTCTTGGGACTCCCTGGACGAAGAGGATCTCGAAGACGTAGAGGAAGAAACTATTGCTAGCGACAAGGCT
  CCCTCATTCAAGAAACCAGTACGGTCACAGCCACCCAAAACCATCCCTCCTTTGCCCCCGCAGCCCTGCT
  CTTTGAAAGCTAGCCGACGGTGGGATACAGTCTCTATCGCCGGCAGTCCGACCGCCCCCGCCGGGAAGCA
  GCCCAAGCGAGCTAGACGCGGTTACTGCAGCTGGCGCGCACATAAGTCCAATATTGTGGCATGTCTTCAA
  CACTGCAGAGGAAACATTAGCTTTGCGAGACGATACTTGCTCTTCCACGACGGGGTGGCCGTGCCACGGA
  ACGTTCTCTACTATTACCGGCATCTGTATAGCCCATACGAAACCTTTGGGGAAAATACTTCCTCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100519 representing NP_040867
  Red=Cloning sites Green=Tags
  
  MPPKGNKHPIAQRQSQQKLQKQWDEEETWDDSQAEEVSDEEAEEQMESWDSLDEEDLEDVEEETIASDKA
  PSFKKPVRSQPPKTIPPLPPQPCSLKASRRWDTVSIAGSPTAPAGKQPKRARRGYCSWRAHKSNIVACLQ
  HCRGNISFARRYLLFHDGVAVPRNVLYYYRHLYSPYETFGENTSSA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 558 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040867
• RefSeq ORF: 558
• Locus ID: 2715920
• MW: 21.3 kDa