E2A (NC_001454) Virus Tagged ORF Clone

CAT#: VC100516

Myc-DDK-tagged ORF clone of viral ORF for single-stranded DNA-binding protein [Human adenovirus F], codon optimized for human cell expression, NP_040864

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100516 represents NCBI reference of NP_040864 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGTAGACAGCAGGAGCTCCCTACTATCACACCTTATCTCCAGGAAACTTCCCCTGAAAGAGCCC
  CTAGCCTGCCACCGAAGAAAAAACTGCGAAAAAACGTCCAGGTCCCGGACAGGGTCCCCGTACTCCCACC
  TTCCCCTGAAGTGGTGCCAGACTCTGAAGAGGAGGAAGAGGAAGTGGTGTACACCGGCTTTTCTCACCCA
  GGCGTTCAGGTGGTTCAGAAGGCCTCCGGAAAGAGGTATGTTAGGCGGCTGGAGCCTAAGGGTGTGCCAC
  CTCCCAGTGAGGAGAACAACGAGGAGGAGGAACCCTCTACGTCCAAGGCCGTGACGAGCGTCGTTTTGAA
  CCCACAGGCGGAACCCCTTGTCTCTGCCTGGGAAAAGGGGATGGATCTGATGATTAAACTCATGGAAAAG
  TACCATGTGGAGGCAGAGGAGAAAAACGGCTTCAAATTTCTGCCCGAACAGTCAAACGTTTATAGAAAGA
  TCTGTCAGACCTGGTTGAACGAAGAGCATCGAGGCCTGCCACTGACATTCACGTCTCATAAAACTTTCGT
  TGAAATGATGGGTAGATTCCTGAGAGCATACGTAGAATCATATGCAGGTGTCAAGAACAATGAGTGGGAG
  CCCACTGGCTGTGCCATCTGGTTGCATGGGTGCACCGAGCAGGAGGGCGTACTGAGGTGCTACCACGGGC
  TGGAAATGATACAGAAAGAACAGTTGGTGGAGATGGACGTTGCCAGTGAGAACGCCCAGCGCGCCCTGAA
  AGAGCACCCCAGTAGGGCGAAAGTTGTACAGAACCGATGGGGTCGATCAGTGGTCCAGCTCAAGAACGAT
  GACGCTCGGTGTTGCGTGGAGGACGTGTCCTGTGCCACCAATGTGTTCTCTGCCAAATCATGCGGACTGT
  TCTTTAGCGAGGGAACCAAAGCTCAGACAGCTTTCCTGCAGATTGAAGCATTTATGCAGGCTGAATACCC
  CAAAATGCAGAACGGTCTGAAAAGATTGCTTATGGTCATGAGGTGTGATTGCCTCTACAAGCCGACCGGG
  GTGCCCCAGCTCGGTAGACAGATGTGTAAAGCCACCCCCTTCGCCCTTAGTAACGTGGACTCCCTGCGGG
  CCGAGGAGGTAACCGATAAGGTGGCTCTCGCCAGCATTCAGTATCCGTGCGTCCTGGTATATCAGTGCGC
  TAATCCCGTCTACCGAAATTCCCGCGGAGGTCAAGGTCCCAATTGCGACTTCAAGATAAGTGCACCCGAC
  CTGCTGGGCGCCCTTCAGTTGGTGCGGCGACTCTGGGGTGAGAACGTTGATGGCCCCCTGCCCAAGATGC
  TGATCCCAGAGTTTAAGTGGAGCTCTAGGTTGCAGTATAGAAATGTTGCCTTGCCTGCCAGCCATGGCGA
  TGGCGAAAAAGAGCCTTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100516 representing NP_040864
  Red=Cloning sites Green=Tags
  
  MAGRQQELPTITPYLQETSPERAPSLPPKKKLRKNVQVPDRVPVLPPSPEVVPDSEEEEEEVVYTGFSHP
  GVQVVQKASGKRYVRRLEPKGVPPPSEENNEEEEPSTSKAVTSVVLNPQAEPLVSAWEKGMDLMIKLMEK
  YHVEAEEKNGFKFLPEQSNVYRKICQTWLNEEHRGLPLTFTSHKTFVEMMGRFLRAYVESYAGVKNNEWE
  PTGCAIWLHGCTEQEGVLRCYHGLEMIQKEQLVEMDVASENAQRALKEHPSRAKVVQNRWGRSVVQLKND
  DARCCVEDVSCATNVFSAKSCGLFFSEGTKAQTAFLQIEAFMQAEYPKMQNGLKRLLMVMRCDCLYKPTG
  VPQLGRQMCKATPFALSNVDSLRAEEVTDKVALASIQYPCVLVYQCANPVYRNSRGGQGPNCDFKISAPD
  LLGALQLVRRLWGENVDGPLPKMLIPEFKWSSRLQYRNVALPASHGDGEKEPF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040864
• RefSeq ORF: 1419
• Locus ID: 2715921
• MW: 53.3 kDa