E3A (NC_001454) Virus Tagged ORF Clone

CAT#: VC100521

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-alpha [Human adenovirus F], codon optimized for human cell expression, NP_040869

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100521 represents NCBI reference of NP_040869 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAATTTGCGTCCTGTTTTGCGTTCTGTCACTGACCTCTTCTCTTCGGACAAGTCCAACAACCGTGG
  GCTCTCTGAGACAGCTGCAGGACTCAACGAAGGGGACCCACCAGACTCTCTATTTCTCCGAATCCACCAC
  CTCAATTGCCCTGAATTGCAGTTGTAGGAATCAACTCGTGCAATGGCGCGCGAACCGGCAGTTCTGCAAG
  TTGTTTTGGGACGCGCTGATTGTCCAGGGCAATAATAGTCTGTGTAACAACTGCACCGCAACAACACTGA
  CCCTGACACCACCCTTTGTTCCAGGACCATACCTGTGTATAGGTACTGGCAGGGGTCCGTCCTGCTTCAA
  CAGATGGACGCTGCAAAAAGAAAATCTGACCACCACGACCCTGCTTCCATTGACAACCTACACTTTCAGC
  CAGAAGAAAAATTACTTTCTCCCAATCATAGCCCTGCTCGCGTTTGTTTGCGTTATTACCGCCAATTATA
  TACTCATCTTCAATCTTGACAATTTCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100521 representing NP_040869
  Red=Cloning sites Green=Tags
  
  MKICVLFCVLSLTSSLRTSPTTVGSLRQLQDSTKGTHQTLYFSESTTSIALNCSCRNQLVQWRANRQFCK
  LFWDALIVQGNNSLCNNCTATTLTLTPPFVPGPYLCIGTGRGPSCFNRWTLQKENLTTTTLLPLTTYTFS
  QKKNYFLPIIALLAFVCVITANYILIFNLDNFY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 519 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040869
• RefSeq ORF: 519
• Locus ID: 2715934
• MW: 19.4 kDa