L1 (NC_001454) Virus Tagged ORF Clone

CAT#: VC100508

Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pIIIa [Human adenovirus F], codon optimized for human cell expression, NP_040856

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100508 represents NCBI reference of NP_040856 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTAGGAGCACCGCCGTGGCCGACGGTTCTCAGCAGGTTAACCCTGCCATGCTGGCCGCACTCCAGT
  CTCAGCCTTCTGGAGTGACCCCTTCTGACGATTGGGCAGCCGCGATGGATAGAATTCTGGCTCTGACCAC
  ACGCAATCCCGAAGCATTCCGCCAACAGCCCCAAGCTAACAGGTTCTCTGCAATTCTGGAAGCTGTGGTA
  CCGTCAAGGACCAATCCCACCCACGAAAAGGTCCTCGCGATCGTTAATGCACTCGTCGAGTCAAAAGCCA
  TCAGGAAGGATGAGGCGGGACTGATCTATAATGCACTCCTGGAACGGGTCGCCAGATATAATTCCACTAA
  CGTTCAGGCCAATTTGGACCGACTGACTACAGATGTGCGCGAAGCCGTTGCCCAGAGAGAAAGGTTCATG
  CACGACGTAAATCTCGGGTCACAGGTTGCATTGAACGCTTTCCTCTCCACACTTCCCGCGAATGTCCCCC
  GGGGGCAGGAAGACTATGTTTCTTTCATTTCTGCACTCCGCCTGCTGGTGGCCGAGGTCCCCCAGTCTGA
  GGTCTACCAGAGCGGCCCCGACTATTTTTTCCAAACCTCCAGACAGGGACTGCAGACTGTCAATTTGACT
  CAAGCGTTTAAAAATCTGCAGGGCATGTGGGGCGTGCGAGCCCCCGTCGGAGACCGCGCCACGATCAGTT
  CTCTGCTGACCCCAAACACCCGGCTCCTCCTTCTCCTTATTGCACCCTTCACCAATTCCAGTACCATTAG
  TCGGGATTCCTATCTTGGCCATCTGATCACATTGTACCAGGAAGCCATCGGACAGACCCAGGTGGATGAG
  CAAACATTCCAGGAGATAACCTCCGTGAGCAGAGCACTGGGGCAGCAGGACACCAGCAGTCTGGAAGCCA
  CACTCAATTTCCTTCTGACTAATAGACGCCAGAAAATTCCTTCTCAGTTCACTCTGAACTCCGAGGAAGA
  GCGCATCTTGCGATACGTGCAACAGAGCGTGTCTCTCTATCTGATGAGGGAGGGAGCAACCGCCAGTTCC
  GCCCTCGATATGACAGCTAGGAACATGGAACCATCCCTGTACTCATCTAATAGACCGTTCATCAATCGAC
  TCATGGACTACTTGCATCGCGCCGCTGCCATGAATTCCGAGTACTTCACAAACGCCATCCTTAATCCACA
  CTGGATGCCTCCTTCAGGATTCTACACCGGAGAGTTCGACGTTCCAGAAGGCGACGACGGATTCCTGTGG
  GATGATGTATCCGAGAGTATTTTCGAGCCCATGCGCTCTCGCAAGAAAGAAGGAGGCGATGAGCTTCCGC
  TGAGTCTCGTAGAAGCCGCTAGTCGAGGCCAGACCCCTGTTCCCAGCCTTCCATCACTGACAAGCTCCAG
  CTCAGGGCGGGTGTTCAGACCACGACTGCCTGGAGAACTGGACTATCTGAGTGACCCCCTTCTGCGCCCC
  GCCCGCAAAAAAAATTTTCCTAACAATGGTGTAGAGAGCCTGGTGGACAAGATGAATCGGTGGAAAACGT
  ATGCTCAAGAGCAGCGAGAGGAGCGCCAACCTAGACCACTGACCGGCACTTTTAGTCGGTGGCGACGGCG
  CGAGGAGGATGCGTATGATTCTGCGGACGACTCTTCAGTGCTGGATCTGGGTGGCACCGGCGCAGCTTCT
  GATCCTTTCGCCCACCTGAGGCCACAGGGGCAGCTCGGCAGACTGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100508 representing NP_040856
  Red=Cloning sites Green=Tags
  
  MLRSTAVADGSQQVNPAMLAALQSQPSGVTPSDDWAAAMDRILALTTRNPEAFRQQPQANRFSAILEAVV
  PSRTNPTHEKVLAIVNALVESKAIRKDEAGLIYNALLERVARYNSTNVQANLDRLTTDVREAVAQRERFM
  HDVNLGSQVALNAFLSTLPANVPRGQEDYVSFISALRLLVAEVPQSEVYQSGPDYFFQTSRQGLQTVNLT
  QAFKNLQGMWGVRAPVGDRATISSLLTPNTRLLLLLIAPFTNSSTISRDSYLGHLITLYQEAIGQTQVDE
  QTFQEITSVSRALGQQDTSSLEATLNFLLTNRRQKIPSQFTLNSEEERILRYVQQSVSLYLMREGATASS
  ALDMTARNMEPSLYSSNRPFINRLMDYLHRAAAMNSEYFTNAILNPHWMPPSGFYTGEFDVPEGDDGFLW
  DDVSESIFEPMRSRKKEGGDELPLSLVEAASRGQTPVPSLPSLTSSSSGRVFRPRLPGELDYLSDPLLRP
  ARKKNFPNNGVESLVDKMNRWKTYAQEQREERQPRPLTGTFSRWRRREEDAYDSADDSSVLDLGGTGAAS
  DPFAHLRPQGQLGRLY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 1728 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040856
• RefSeq ORF: 1728
• Locus ID: 2715925
• MW: 64.3 kDa