L5 (NC_001454) Virus Tagged ORF Clone

CAT#: VC100527

Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus F], codon optimized for human cell expression, NP_040875

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100527 represents NCBI reference of NP_040875 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGGACCCGCATCGAAGACGACTTTAATCCGGTTTATCCCTACGACACTAGTAGTACCCCAAGTA
  TACCATACGTCGCGCCACCTTTCGTGTCCTCCGACGGACTCCAAGAAAACCCCCCAGGCGTGCTGGCCCT
  GAAATACACTGATCCTATCACCACAAACGCCAAACATGAGTTGACCCTGAAACTTGGATCTAATATCACA
  CTGCAAAATGGCCTGTTGAGCGCCACAGTGCCTACGGTGTCTCCGCCACTCACTAACAGCAACAATAGTC
  TGGGCTTGGCAACAAGCGCACCTATTGCTGTCTCAGCCAATTCTCTTACGCTGGCCACAGCAGCACCGCT
  GACCGTCTCCAATAACCAGCTGTCCATCAATACCGGAAGGGGACTGGTTATTACGAATAACGCAGTGGCC
  GTGAACCCAACTGGGGCTCTCGGATTCAATAATACCGGCGCACTGCAGCTCAATGCGGCAGGCGGCATGC
  GCGTTGACGGTGCCAATCTTATACTCCACGTGGCATATCCTTTCGAAGCGATCAACCAGCTGACACTTAG
  ACTCGAGAATGGACTCGAGGTAACCAATGGCGGGAAGCTCAACGTGAAGCTGGGGAGCGGTCTGCAGTTC
  GATAATAATGGCCGGATCACAATCAGTAACCGGATCCAGACCCGAGGCGTGACATCTTTGACAACAATCT
  GGAGCATTTCCCCCACCCCCAACTGTAGCATTTATGAAACACAGGACGCCAACCTTTTCCTCTGCCTCAC
  TAAAAATGGCGCGCATGTGCTGGGAACCATCACGATTAAAGGTCTGAAGGGAGCGCTTAGAGAGATGAAT
  GACAACGCCTTGTCAGTGAAACTCCCTTTCGATAATCAGGGCAACTTGCTGAATTGTGCTTTGGAAAGCA
  GCACCTGGCGATACCAGGAGACTAACGCGGTGGCCAGCAACGCCCTCACGTTTATGCCAAACAGTACTGT
  CTATCCGCGAAACAAGACCGCCGACCCGGGCAATATGTTGATCCAGATATCACCAAATATTACCTTCTCA
  GTTGTATATAATGAGATAAATTCTGGTTATGCATTTACTTTTAAGTGGTCAGCGGAACCGGGGAAACCAT
  TCCACCCACCGACAGCCGTGTTCTGTTACATAACAGAGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100527 representing NP_040875
  Red=Cloning sites Green=Tags
  
  MKRTRIEDDFNPVYPYDTSSTPSIPYVAPPFVSSDGLQENPPGVLALKYTDPITTNAKHELTLKLGSNIT
  LQNGLLSATVPTVSPPLTNSNNSLGLATSAPIAVSANSLTLATAAPLTVSNNQLSINTGRGLVITNNAVA
  VNPTGALGFNNTGALQLNAAGGMRVDGANLILHVAYPFEAINQLTLRLENGLEVTNGGKLNVKLGSGLQF
  DNNGRITISNRIQTRGVTSLTTIWSISPTPNCSIYETQDANLFLCLTKNGAHVLGTITIKGLKGALREMN
  DNALSVKLPFDNQGNLLNCALESSTWRYQETNAVASNALTFMPNSTVYPRNKTADPGNMLIQISPNITFS
  VVYNEINSGYAFTFKWSAEPGKPFHPPTAVFCYITEQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 1161 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001454.1, NP_040875
• RefSeq ORF: 1161
• Locus ID: 2715923
• MW: 41.3 kDa