E4 ORF6/7 (AC_000019) Virus Tagged ORF Clone

CAT#: VC100105

Myc-DDK-tagged ORF clone of viral ORF for E4 ORF6/7 [Human adenovirus 35], codon optimized for human cell expression, AP_000602

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4 ORF6/7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100105 represents NCBI reference of AP_000602 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGCAGTAACAGCATTATGACCAGACTCAGAGCTCGGAGCACAAGTTGTGCGAGGCATCACCCGT
  ATACCCGGGCTCAGCTGCCTCGGTGTGAGGAAAACGAGACCCGGGCTAGTATGACCGAAGATCACCCACT
  GCTGCCTGATTGTGATACGATGACTATGCACAGTATGACCGTCATTCAGACCCCTGAATCACATCCGCAG
  CAGCTCGATTGTGAGAGTGCACTCAAGGATTACAGAGATGGGTTTCTTTCCATTACGGACCCCAGGCTCG
  CCCGCTCAGAAACCGTGTGGAACGTGGAAAGCAAGACGATGTCAATTAGTAACGGGATCCAGATGTTTAA
  GGCCGTGAGGGGCGAGCGACTGGTGTACAGTGTAAAATGGGAAGGCGGGGGCAAGATCACCACCAGGATC
  CTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100105 representing AP_000602
  Red=Cloning sites Green=Tags
  
  MSGSNSIMTRLRARSTSCARHHPYTRAQLPRCEENETRASMTEDHPLLPDCDTMTMHSMTVIQTPESHPQ
  QLDCESALKDYRDGFLSITDPRLARSETVWNVESKTMSISNGIQMFKAVRGERLVYSVKWEGGGKITTRI
  L
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 423 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000602
• RefSeq ORF: 423
• MW: 16.0 kDa