pTP (AC_000019) Virus Tagged ORF Clone

CAT#: VC100079

Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus 35], codon optimized for human cell expression, AP_000577

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pTP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100079 represents NCBI reference of AP_000577 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTTTCTATTAATGACTGCGCCAGACTGACCGGGCAGACTGTGCCTACCATGAATTATTTCATGC
  CTCTTCGCAATATATGGAACCGAGTTCGGGAATTCCCCAGAGCTTCAACGACCGCAGCAGGAATTACATG
  GATGTCAAGATACATATATGGCTACCATCGCCTGATGTTGGAGGATCTGGCGCCAGGCGCGCCTGCCACC
  GAAAGATGGCCCCTGTATCGACAGCCCCCCCCTCACTTCCTTATTGGGTATCAGTACCTTGTGCGCACCT
  GCAATGACTACATCTTCGACACCCGGGCCTACAGTAGACTCAAATACACAGAACTGGTGAGACCTGGTCA
  CCAAACAGTAAATTGGTCCGTAATGGCCAATTGCGCTTACACCATAAATACGGGTGCCTACCACAGGTTC
  GTTGACTTTGACGATTTTCAAGCCACGTTGACACAAGTGCAGCAAGCCATATTGGCAGAACGGGTCGTGG
  CCGACTTGGCCTTGGTTCAGCCTATGCGGGGGTTCGGCCTGACTAGAATGCATGGCCGGACTGGAGAGGA
  AGAAGTACCTGTAGAACGATTGATGCAGGATTACTACAAGGACCTGGCCCGATGCCAGGACAATGCTTGG
  GGGATGGCAAACAGGCTGCGGATCCAGCAAGCCGGACCTAAGGATCTTGTGCTGCTGGCTACAATTCGGA
  GATTGAGGACTGCATACTTTAACTTTATAACTTCCTCCATCGTATCTCCATCACGCGACGAAGAGGAAAC
  TGAGGACCCCGACAGGACCCATAACCATCCCAGGCCACAGGAAGCAGTTCTGTCCCTGCCATGTGATTGT
  GATTGGCTGGATGCTTTCGTGGAGCGATTCAGTGATCCAGTTGATCTTGATACAATCCGAAGTTTGCGGG
  CCGTACCTACCGGCCAGCTGATTAAGTGCATAATTTCAGCCCTGAGCCTGCCCAACGGAGAGCCGCCATC
  TCACCACTTTAAGGAGATGCGGGGTGGCGTATTTACGCTTCGGCCCCGCGAGAATGGAAGGGCTGTGACT
  GAGACCATGAGGCGCAGGAGGGGGGAGGTCATAGAGCGCTTCATAGACAGGCTCCCAGTTCGAAGGAGAA
  GACGGCGGGTCCCCCCTCCACCTCCTCCAGAGGATGAGGAGGAAATGCTCGTAGAGGAAGAGATCGAGGA
  GGAGGAGGAAATACCTGGGGCATTCGAAAGAGAAGTGCGCGCAACTATTGCCGAGCTGATCCGGCTGCTC
  GAAGAGGAGCTCACCGTCTCCGCTCGCAACAGTCAGTTTTTTAATTTTGCTGTAGATTTTTATGAGGCTA
  TGGAACGGCTGGAGGCACTGGGCGACGTGTCAGAGATGCCACTGAGGAGGTGGATCATGTACTTTTTCGT
  AGCAGAACACATTGCCACAACGCTCAATTATCTTTTTCAACGGCTTTGCAATTACGCAGTATTTACGCGC
  CACGTTGAACTGAATTTGGCGCAAGTTGTGATGCGAGCCAGGGACTCAGAAGGCGCTGTAGTTTATAGCC
  GAGTCTGGAACGAGGCCGGTATGAATGCCTTTTCCCAGCTTATGGGCAGAATCTCCAACGACCTGGCAGC
  CACCGTGGAGCGCGCAGGTCGAGGTGACCTGCAGGAAGAGGAAATAGAACAGTTCATGGCCGAGATAGCT
  TATCAGGACAACTCCGGCGATGTCCAGGAAATCCTCAGGCAGGCTGCCGTCAACGATACAGAGATCGACT
  CCGTGGAACTGAGTTTTCGCTTCAAACTGACTGGTCCTGTCGCCTTTACTCAGAGGCGACAGATCCAAGA
  CGTAAACAGGAGGGTGGTGGCGCACGCCAGTCTGCTGCGGGCTCAGTATCAGAACCTTCCCGTTCGCGGA
  GCCGATGTCCCTCTCCCTGCCATGCCCCCTGGGCCGGAGCAACCCTTGCCACCAGGCGCTCGCCCCAGGC
  ACCGCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100079 representing AP_000577
  Red=Cloning sites Green=Tags
  
  MALSINDCARLTGQTVPTMNYFMPLRNIWNRVREFPRASTTAAGITWMSRYIYGYHRLMLEDLAPGAPAT
  ERWPLYRQPPPHFLIGYQYLVRTCNDYIFDTRAYSRLKYTELVRPGHQTVNWSVMANCAYTINTGAYHRF
  VDFDDFQATLTQVQQAILAERVVADLALVQPMRGFGLTRMHGRTGEEEVPVERLMQDYYKDLARCQDNAW
  GMANRLRIQQAGPKDLVLLATIRRLRTAYFNFITSSIVSPSRDEEETEDPDRTHNHPRPQEAVLSLPCDC
  DWLDAFVERFSDPVDLDTIRSLRAVPTGQLIKCIISALSLPNGEPPSHHFKEMRGGVFTLRPRENGRAVT
  ETMRRRRGEVIERFIDRLPVRRRRRRVPPPPPPEDEEEMLVEEEIEEEEEIPGAFEREVRATIAELIRLL
  EEELTVSARNSQFFNFAVDFYEAMERLEALGDVSEMPLRRWIMYFFVAEHIATTLNYLFQRLCNYAVFTR
  HVELNLAQVVMRARDSEGAVVYSRVWNEAGMNAFSQLMGRISNDLAATVERAGRGDLQEEEIEQFMAEIA
  YQDNSGDVQEILRQAAVNDTEIDSVELSFRFKLTGPVAFTQRRQIQDVNRRVVAHASLLRAQYQNLPVRG
  ADVPLPAMPPGPEQPLPPGARPRHRF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 1968 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000577
• RefSeq ORF: 1968
• MW: 75.6 kDa