DBP (AC_000019) Virus Tagged ORF Clone

CAT#: VC100089

Myc-DDK-tagged ORF clone of viral ORF for DBP [Human adenovirus 35], codon optimized for human cell expression, AP_000587

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100089 represents NCBI reference of AP_000587 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCCCGGGAGGGGAATCAGCTGAGTGACCGGCATCGCGAGCATACGCCCGAGAGAGGCAGGGGAA
  GTGCCAGTCACCCCCCCTCACGCTCCGATCGGTCCCCTTCCCAATCTCCACCGCCACTGCCTCCCAAGAA
  AAATACTTGCAGGCGGGTGGGAAGTGGTTCCAGTACCGATAGCCAGCTTGTTATGGTTTCCGAGACGAGC
  CAAAGTTCTCTTTCTCCCGAGCGGAGTGACTCACCACCTCCTCCAATCCCCCCCAAGAAGAAGCCAAGAA
  AGACCAAGCACATACCCATGCAGGACATCTCCCAGGACTCCGAAGAAGAGAGGGAGGAGGCACAATTGGT
  GGCCGTAGGGTTCTCATATCCACCCGTCAGAATTGTCGAAAAGGATGGGAAGAGGTCTATCGAGAAAATC
  GCTAAAGATGATCCCCTGGCTAAGGGCGCCGCTGCATGCACTGTGAAGAACCCCATCAGCCTGCCTTTGG
  TTTCTGCATGGGAAAAAGGCATGGAAGTAATGTGCTTGCTCATGGAGAAGTATCGGCTCGATAATGAGCT
  GCGCACATGTTTTAAATTGATGCCCGAACAGCACGAACAGTACAAACGGATTTGCCATCAATACGTGAAC
  GAGGAGCACCGAGGAATCCAACTGACTTTCACATCTCACAAGACCCTGAGCACAATGATGGGGAGATTCC
  TGCAGGGCATGATCCATTCATTTAGCCAAATCGCCCATCACAACTGGGAGTGTACAGGCTGCGCCTTGTG
  GCCCCACGGGTGCAACGACTATGAGGGGAAACTGAAGTGTCTTCACGGGAACATAATGATACAGAAGGAG
  CAAATTATCGAGATGGATGTTGCTTCTGAGAATGGACAGAGGGCTCTCAAAGAAAACCCTGAACGAACAA
  AGATTACTCAGAATCGGTGGGGCAGGAGTGTGGTGCAGATCGCCAACAATGATGCCAGATGTTGTGTTAA
  CGACGCTGGATGCGCTGCTAATCAGTTCTCCAGTCGCTCTTGTGGCATGTTTTACACGGAAGGATCTAAA
  GCACAACAAGCCTTCAAGCAATACGATGCATTTATGCGCGCCGTATACCCCGGCATACGGCAGGACCAGG
  CCAAGATGATCCTGATTCCACTGCATTGCGACTGCAATCACAAACCCAACTGGGTGCCCGCCATGGGCAG
  ACAGACGTGCAAAATGACACCATTTAGTATCGCTAACGCGGAAGACCTGGATGTGGGGATGATTGCAGAC
  CCCACCGTCCTGGCTAGTGTGAGGCATCCATCCCTTATGGTATTCCAATGTTGTAACCCCGTATACAGAA
  ATAGTAGGGCACAGTCCACAGGGCCAAATTGTGATTTTAAGATCTCCGCACCCGACCTGCTTGGCGCTCT
  CCAGCTCACACGAAAGCTTTGGTCTGATATCCTGCCCGATATTCCTGTTCCTAAGCTGGTAATCCCTGAA
  TTCAAATGGCAGCCTAAGTATCAATTTCGGAACGTGTCCCTCCCAGCCGGCCACAGCGACTCTCGCCAGA
  ACCCCTTTGATTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100089 representing AP_000587
  Red=Cloning sites Green=Tags
  
  MASREGNQLSDRHREHTPERGRGSASHPPSRSDRSPSQSPPPLPPKKNTCRRVGSGSSTDSQLVMVSETS
  QSSLSPERSDSPPPPIPPKKKPRKTKHIPMQDISQDSEEEREEAQLVAVGFSYPPVRIVEKDGKRSIEKI
  AKDDPLAKGAAACTVKNPISLPLVSAWEKGMEVMCLLMEKYRLDNELRTCFKLMPEQHEQYKRICHQYVN
  EEHRGIQLTFTSHKTLSTMMGRFLQGMIHSFSQIAHHNWECTGCALWPHGCNDYEGKLKCLHGNIMIQKE
  QIIEMDVASENGQRALKENPERTKITQNRWGRSVVQIANNDARCCVNDAGCAANQFSSRSCGMFYTEGSK
  AQQAFKQYDAFMRAVYPGIRQDQAKMILIPLHCDCNHKPNWVPAMGRQTCKMTPFSIANAEDLDVGMIAD
  PTVLASVRHPSLMVFQCCNPVYRNSRAQSTGPNCDFKISAPDLLGALQLTRKLWSDILPDIPVPKLVIPE
  FKWQPKYQFRNVSLPAGHSDSRQNPFDL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 1554 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000587
• RefSeq ORF: 1554
• MW: 58.3 kDa