pol (AC_000019) Virus Tagged ORF Clone

CAT#: VC100078

Myc-DDK-tagged ORF clone of viral ORF for pol [Human adenovirus 35], codon optimized for human cell expression, AP_000576

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pol
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100078 represents NCBI reference of AP_000576 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTCGTACAGGCGCACGGGGCCGGTTCCTTCCACCCCGAGACAAGCGACACTCGGAGGCAATCCA
  CGCGGGGAGGCGCGAGAAAATCTTCACAGAGTGCAATTCCAGAGCCGGCCCGCGCTAGACGCCGCCGCGC
  GACCACAAGTCATGCCCCAGGCAGTGGTACTACCGCTGCCGCTCGAAGAGCCAGCCCCTCACCTCTTCTG
  TCAATGCAGGAAGCCCGCGAGCCCCCCCCAATCAAGCGGAAGAACAAGGGCACCGTTGTCGCTCCCAAGG
  GTCACGGCACACTCCAGGCTATCGACGTGTCCACTAATGAGCCCGTGGAGATCAAATACCACCTGAACCT
  TCCTCACGCCCTGGAAAAGATTATGCAGGTCAATCTGCTGTCTCTTCCCACAAATCTGTCCCCCCAGCGC
  CTCCGCACCCTGGATAGTAGTAGCCTTCGGGCACTGGTTCTTCAGCTCCGGCCTTCCAGGGCTGAGGTTT
  GGACATGCCTTCCACGGGGACTCGTGAGCATGACGACAATTGAGACCGAGGACGAGCAGGCCGACACTGC
  GAATATTGAGGAACATGAGATTCAGTCCCCAGGACTCGGTTTCCCCCTGAAGTTCCTGGTGAAGGGTTCC
  CAAGTCCAGCTTATTCACGAGATACAGCCTGTGCAGAGATGTGACTACTGCGGCAGATTGTACAAACATA
  AACATGAGTGCTCTGCACGGCGGCGGAATTTTTACTTTCATCACATCAATTCCCAGTCAAGTAACTGGTG
  GCAGGAAATACAGTTTTTCCCCATCGGGTCCCACCCAAGGACGGAAAGACTCTTCTTGACATATGATGTG
  GAGACTTACACGTGGATGGGCTCCTTCGGGAAGCAGCTTGTCCCCTTCATGTTGGTCATGAAATTGTCTG
  GAGAAGAGCGACTCGTCAACATGGCACACGACCTTGCAATAAAGCTTAAATGGGATAGGTGGCAGCAGGA
  TCCGCGGACCTTCTACTGTGTTACGCCGGAGAAGATGGCTATTGGACGCCACTTTCGCCAGTATCGGGAT
  CAGCTGCAGACTGCGTTGGCCGTCGATCTCTGGTCATCATTTCTGCACGCCAACCCACACCTGCATGAAT
  GGGCGCTGGAACACTACGCCCTGAGCGACCCTACGGATCTGACTTTCGATGAACTCAGTAAGCTGCCTCA
  CGTGCGGGGCACCCCACGATTTATCGAGCTGTACATTGTCGGACACAATATCAATGGATTTGATGAAATT
  GTCCTCGCAGCACAAGTGATAAATAATCGCGCAGAGGTGCCTCAGCCTTTCCGCATTACTAGAAACTTTA
  TGCCTAGAGCTGGGAAAATTCTGTTTAATGATGTGACGTTCGCTCTGCCCAACCCCGCCTACAAAAAGAG
  AGTCGATTTCCAGCTGTGGGAGCAAGGCGCATGTGACGATATGGACTTCAAATACCAGTTTCTGAAAGTT
  ATGGTAAGGGATACGTTCGCACTCACCCATGCCTCACTGAGGAAGGCTGCACAGGCCTACTCTCTGCCCG
  TAGAAAAAGGCTGTTGCCCTTATAAAGCCGTTAATCAGTTCTACATGCTGGGGTCATATAGAGCCGATAA
  GGACGGGTTCCCGCTTGAAGAGTACTGGAAGGATCGCGAGGAATACCTGTTGAACCGGGAGCTGTGGGAA
  AAAAAGAATCAGCCCGGATATGATATCATCCAGGAAACTCTGGACTATTGTGCACTTGATGTTCTCGTTA
  CCGCAGAGCTGGTTACTAAACTGCAGGAATCCTATGCTCATTTCGTCAGGGACAGCGTGGGACTTCCACA
  CGCCCACTTTAACATTTTTCAGAGACCGACTATAAGCTCCAACTCACACGCCATTTTCAGGCAAATCGTA
  TATCGCACCGAGAAGCCTAATAGGACAAACCTTGGCCCCGGGCTGTTGGCCCCAAGCCACGAGCTCTATG
  ACTACGTTCGGGCTTCTATAAGAGGAGGGCGCTGTTACCCTACTTATATCGGAATCTTTGAGGACCCCAT
  TTATGTTTACGACATCTGCGGCATGTACGCCTCCGCCCTGACCCACCCAATGCCTTGGGGCAGCCCGCTG
  AATCCGTACGAAAGAGCCCTGGCCGCCCGAGAATGGCAGATGGCTTTGGACGATCCTACCCCTATCTCTT
  ATTTCGACAAAGATCTGCTGCCCGGCATTTTCACTATGGATGCAGATCCCCCCGATGAGTTGATGCTGGA
  CCCCCTCCCACCGTTTTGTTCACGCAAGGGGGGACGCCTTTGCTGGACAAACGAGCCCTTGCGCGGTGAA
  GTGGCAACATCCGTAGATCTGATTACGCTGCATAACCGAGGTTGGCAGGTACGCATTGTGCCAGACGAAC
  TCACAACAATTTTTCCCGAATGGAAATGCCTGGCCCGAGAGTACGTGCAGCTCAATATAGCAGCTAAAGA
  GAGAGCAGACAAAGAGAAAAATCAGACAATGCGGTCCATCGCCAAGCTCCTTAGTAACGCTCTGTATGGG
  AGCTTCGCCACAAAATTGGACAATAAAAAAATTGTATTTAGCGACCAGATGGATGAAAACCTTATGAAAG
  GCATCTCCACCGGTACTGTGAACATTAAGAGTAGCAGCTTCCTTGAGACCGATAACCTGAGCGCCGAAGT
  AATGCCCGCTTTCGAACGGGAATACCTGCCCCAGCAGCTGACCTTTTTGGACTCAGATCCAGAGGACTCA
  GAGGAAGAGCAGAGAACCGCACCGTTTTATACTCCGCCAGCCGGGACCCCAGGACACGTTAAGTATACGT
  ACAAGCCCATTACTTTCCTGGATGTTGAGGAGGGGGATATGTGTCTGCACACACTCGAAAAAGTGGATCC
  CCTGGTGGACAACGACAGATACCCAAGCCATATAGCAAGTTTTGTCCTGGCGTGGACAAGAGCTTTCGTG
  AGCGAGTGGTCTGAGTTTCTGTACGAAGAGGATAGAGGCACCCCGCTGGAGAAGCGGCCGGTGAAGTCAG
  TGTACGGCGATACGGATTCACTGTTCGTAACACAGCGGGGCCATGAGCTCATGGAAACAAAAGGCAAGAA
  GCGGATCAAAAAATACGGGGGGAAGCTGGTGTTCGATCCAAACGAACCTGACCTCACCTGGTTGGTGGAG
  TGCGAAACCGTCTGCGCCAGCTGCGGAGCCGACGCATACTCACCTGAGAGTATCTTCCTGGCTCCTAAAC
  TGTACGCCCTTAAATGTCTTTATTGCCCAGTTTGCAAAAAAACATCTAAAGGTAAGCTGCGGGCAAAGGG
  GCACGCTGCCGAGGCACTTAACTACGAGCTTATGGTGAATTGCTACCTGGCTGATATACAGGGGGCCAAA
  CGACAGAAGTTTTCTACCAGCAGAATGTCATTGAAGAGAACTCTCGCATCAGCCCAAGCCGGTGCACACC
  CCTTTACAGTGACCGAAACTACCCTTACTCGCACACTCAGACCATGGAAAGATCGGACGCTGGCCGCCCT
  TGACGCCCACCGACTGATCCCCTATTCCCGCTCCCGCCCTAATCCACGGAACGAGGAAGTGTGTTGGATC
  GAAATGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100078 representing AP_000576
  Red=Cloning sites Green=Tags
  
  MALVQAHGAGSFHPETSDTRRQSTRGGARKSSQSAIPEPARARRRRATTSHAPGSGTTAAARRASPSPLL
  SMQEAREPPPIKRKNKGTVVAPKGHGTLQAIDVSTNEPVEIKYHLNLPHALEKIMQVNLLSLPTNLSPQR
  LRTLDSSSLRALVLQLRPSRAEVWTCLPRGLVSMTTIETEDEQADTANIEEHEIQSPGLGFPLKFLVKGS
  QVQLIHEIQPVQRCDYCGRLYKHKHECSARRRNFYFHHINSQSSNWWQEIQFFPIGSHPRTERLFLTYDV
  ETYTWMGSFGKQLVPFMLVMKLSGEERLVNMAHDLAIKLKWDRWQQDPRTFYCVTPEKMAIGRHFRQYRD
  QLQTALAVDLWSSFLHANPHLHEWALEHYALSDPTDLTFDELSKLPHVRGTPRFIELYIVGHNINGFDEI
  VLAAQVINNRAEVPQPFRITRNFMPRAGKILFNDVTFALPNPAYKKRVDFQLWEQGACDDMDFKYQFLKV
  MVRDTFALTHASLRKAAQAYSLPVEKGCCPYKAVNQFYMLGSYRADKDGFPLEEYWKDREEYLLNRELWE
  KKNQPGYDIIQETLDYCALDVLVTAELVTKLQESYAHFVRDSVGLPHAHFNIFQRPTISSNSHAIFRQIV
  YRTEKPNRTNLGPGLLAPSHELYDYVRASIRGGRCYPTYIGIFEDPIYVYDICGMYASALTHPMPWGSPL
  NPYERALAAREWQMALDDPTPISYFDKDLLPGIFTMDADPPDELMLDPLPPFCSRKGGRLCWTNEPLRGE
  VATSVDLITLHNRGWQVRIVPDELTTIFPEWKCLAREYVQLNIAAKERADKEKNQTMRSIAKLLSNALYG
  SFATKLDNKKIVFSDQMDENLMKGISTGTVNIKSSSFLETDNLSAEVMPAFEREYLPQQLTFLDSDPEDS
  EEEQRTAPFYTPPAGTPGHVKYTYKPITFLDVEEGDMCLHTLEKVDPLVDNDRYPSHIASFVLAWTRAFV
  SEWSEFLYEEDRGTPLEKRPVKSVYGDTDSLFVTQRGHELMETKGKKRIKKYGGKLVFDPNEPDLTWLVE
  CETVCASCGADAYSPESIFLAPKLYALKCLYCPVCKKTSKGKLRAKGHAAEALNYELMVNCYLADIQGAK
  RQKFSTSRMSLKRTLASAQAGAHPFTVTETTLTRTLRPWKDRTLAALDAHRLIPYSRSRPNPRNEEVCWI
  EMP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 3579 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000576
• RefSeq ORF: 3579
• MW: 136.3 kDa