V (AC_000019) Virus Tagged ORF Clone

CAT#: VC100084

Myc-DDK-tagged ORF clone of viral ORF for V [Human adenovirus 35], codon optimized for human cell expression, AP_000582

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: V
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100084 represents NCBI reference of AP_000582 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAGCGCAAGTATAAGGAGGAAATGTTGCAGGTCATAGCTCCCGAAGTTTATGGTCAGCCCCTGA
  AAGACGAGAAGAAACCCCGGAAAATCAAGAGGGTTAAAAAGGACAAAAAAGAAGAAGAAGACGGCGATGA
  CGGGCTTGCCGAGTTCGTGCGGGAATTCGCTCCACGAAGGAGGGTGCAGTGGAGAGGCAGAAAGGTGCGC
  CATGTGCTGAGACCAGGGACGAGCGTAGTCTTCACGCCAGGTGAGAGAAGCTCCGCCACATTCAAGAGGT
  CATATGACGAGGTTTATGGAGATGACGATATACTGGAGCAGGCAGCCGACCGCCTGGGCGAGTTCGCCTA
  CGGCAAGCGCTCACGCATCACTTCAAAGGATGAAACCGTCTCCATCCCTTTGGATCACGGTAATCCCACC
  CCGAGCTTGAAGCCAGTGACTCTGCAGCAAGTGCTGCCTGTTACACCTCGAACGGGTGTCAAGCGGGAAG
  GGGAGGATTTGTACCCCACCATGCAGCTGATGGTCCCCAAGAGACAGAAACTTGAGGACGTGCTTGAGAA
  AGTGAAGGTGGATCCGGATATCCAGCCCGAAGTCAAGGTGAGACCCATCAAACAGGTAGCACCCGGGCTG
  GGGGTACAGACGGTGGACATAAAGATCCCCACGGAGTCCATGGAGGTGCAGACTGAGCCGGCCAAACCGA
  CTGCAACAAGTACTGAAGTGCAGACAGACCCTTGGATGCCAATGCCTATTACAACAGACGCCGCCGGCCC
  TACCAGGCGGAGCCGCCGCAAGTATGGCCCCGCTAGCCTGCTCATGCCAAACTACGTCGTTCACCCATCC
  ATCATTCCCACCCCAGGTTACAGAGGCACTCGCTACTATAGATCCAGGAACTCTACCAGTCGGAGGAGAA
  GAAAAACCCCCGCAAATCGGTCTCGGCGGAGACGCAGAACGTCAAAGCCTACCCCCGGCGCTCTGGTGCG
  ACAAGTGTACAGAAATGGGTCTGCTGAGCCGCTCACTCTGCCCAGGGCAAGATATCACCCTAGCATAATT
  ACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100084 representing AP_000582
  Red=Cloning sites Green=Tags
  
  MSKRKYKEEMLQVIAPEVYGQPLKDEKKPRKIKRVKKDKKEEEDGDDGLAEFVREFAPRRRVQWRGRKVR
  HVLRPGTSVVFTPGERSSATFKRSYDEVYGDDDILEQAADRLGEFAYGKRSRITSKDETVSIPLDHGNPT
  PSLKPVTLQQVLPVTPRTGVKREGEDLYPTMQLMVPKRQKLEDVLEKVKVDPDIQPEVKVRPIKQVAPGL
  GVQTVDIKIPTESMEVQTEPAKPTATSTEVQTDPWMPMPITTDAAGPTRRSRRKYGPASLLMPNYVVHPS
  IIPTPGYRGTRYYRSRNSTSRRRRKTPANRSRRRRRTSKPTPGALVRQVYRNGSAEPLTLPRARYHPSII
  T
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 1053 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000582
• RefSeq ORF: 1053
• MW: 40.1 kDa