100K (AC_000019) Virus Tagged ORF Clone

CAT#: VC100090

Myc-DDK-tagged ORF clone of viral ORF for 100K [Human adenovirus 35], codon optimized for human cell expression, AP_000588

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 100K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100090 represents NCBI reference of AP_000588 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACTCAGCCCTTGTTGAGCACCAGCCCCCGGGTACCCTCACACCTGGTACTTAGCGATGAAGAAA
  AGGAACAGTCTCTCTCAATCCCCCCACCCAGTCCCGCTACCACTTCTACTCTGGAGGACAAGGAAGTGGA
  TGCCTCTCACGATATGCAGAATAAAAAAGCCAAAGAGTCAGAGACCGATATTGAACAGGACCCAGGATAC
  GTAACCCCGGTGGAGCATGAGGAAGAGCTGAAAAGATTCCTGGAACGGGAGGACGAAAATTGTCCCAAAC
  AGAGAGCCGATAACTACCACCAAGACGCTGGTAACAGAGATCAGAATACGGATTACTTGATCGGGCTGGA
  TGGAGAAGACGCCCTCTTGAAACACCTGGCTAGGCAGTCTCTGATCGTTAAGGATGCCTTGCTTGACCGA
  ACCGAAGTGCCGATTAGTGTTGAGGAGCTCTCCTGTGCCTACGAGCTGAATCTGTTTTCACCAAGGACAC
  CCCCCAAGAGACAGCCCAATGGGACCTGCGAACCGAATCCCAGGCTCAACTTTTACCCCGCATTCGCTGT
  TCCAGAGGTCCTCGCAACGTACCACATCTTTTTTAAAAATCAGAAGATCCCTGTGAGTTGTAGGGCTAAC
  AGAACCCGCGCGGATGCTCTGCTTAATCTGGGTCCAGGGTCTAGGCTGCCGGATATTGCCTCTCTGGAGG
  AGGTGCCTAAGATTTTTGAAGGTCTCGGTAATAACGAGACAAGAGCAGCTAATGCCTTGCAGAAGGGCGA
  AAACGGCATGGATGAGCACCACAGTGTGCTGGTTGAACTGGAAGGAGACAACGCAAGACTGGCAGTGCTC
  AAAAGGTCAGTAGAGGTCACACACTTCGCCTATCCTGCAGTCAATCTTCCTCCCAAGGTGATGACAGCCG
  TCATGGACCAGTTGTTGATTAAGCGCGCATCACCACTCAGCGAAGATATGCATGATCCGGACGCCTGTGA
  CGAAGGCAAGCCAGTCGTGAGCGATGAGCAGCTGACACGGTGGCTGGGAACCGATTCTCCACGCGACCTG
  GAAGAACGGCGCAAACTCATGATGGCAGTAGTGCTTGTCACCGTGGAACTGGAGTGCCTGAGGCGATTTT
  TCACCGACTCCGAGACCTTGCGCAAACTGGAAGAGAATCTTCACTACACCTTTAGACACGGATTTGTCCG
  ACAGGCATGTAAGATCTCCAATGTGGAACTGACAAACCTGGTTTCATACATGGGTATTCTGCACGAAAAT
  CGCTTGGGCCAGTCAGTACTCCATAGCACCCTGAAGGGGGAGGCACGGAGAGACTATATCCGCGATTGTG
  TATATCTGTACCTCTGCCATACATGGCAGACTGGCATGGGTGTGTGGCAGCAATGTCTGGAAGAACAGAA
  CCTCAAGGAACTTGACAAGCTGCTTCAGAAGAGCCTGAAGGTTCTCTGGACGGGATTTGATGAGCGAACT
  GTGGCCTCTGACCTGGCTGATCTCATCTTCCCCGAAAGGCTCCGGGTGACCCTCCGCAATGGTCTGCCAG
  ATTTTATGAGTCAGAGCATGCTGAATAACTTCCGGTCTTTTATCCTCGAACGCTCAGGTATACTGCCGGC
  CACATGCTGCGCTTTGCCATCTGATTTTGTCCCCCTGACCTATCGGGAGTGCCCCCCCCCTCTGTGGTCC
  CACTGTTATTTGTTCCGACTGGCTAACTACCTGTCCTATCACTCAGACGTAATTGAAGACGTGTCTGGCG
  ATGGACTGCTGGAATGCCACTGTCGCTGTAATTTGTGCACTCCGCACCGGTCTCTCGCGTGCAACCCCCA
  GCTCATGTCCGAGACACAGATTATTGGGACCTTCGAGCTCCAGGGGCCATCCTCCCAGGGGGACGGGTCC
  AGCCCTGGGCAGTCCCTGAAGCTTACCCCCGGGCTGTGGACGTCTGCTTACTTGAGAAAATTTGCACCTG
  AGGATTATCATCCATATGAGATAAAATTTTACGAAGATCAGTCACAGCCCCCGAAAGCAGAGTTGTCTGC
  TTGCGTCATCACGCAAGGTGCCATCCTTGCTCAGCTCCAAGCGATTCAGAAGTCTCGCCAGGAGTTTCTG
  TTGAAGAAGGGCAAAGGCGTCTACTTGGATCCACAGACTGGAGAGGAACTCAATACACGGTTTCCACAGG
  ATGTGCCAACAACCCGGAAACAGGAGGTGGAGGGGGCTGCCGCCGCTCCCCGCAGGTATGGTGGCAGACT
  TGGCCAAAGCGGGAGAGGAGGGGGAGGCGGGCAATCTGGAGGACAGTCTGGTGGACGACAATTTGGTGGA
  GGCAAACGCGGCGGCCGGGGAGGTGGACGGTCTAATAGAAGACAGACGGTTATCTTGGGATGCGGAGATA
  AACAACAGAGGTATCACTTGAGGTCCGAATCCAGAAACCCAGCGGCAAGCCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100090 representing AP_000588
  Red=Cloning sites Green=Tags
  
  METQPLLSTSPRVPSHLVLSDEEKEQSLSIPPPSPATTSTLEDKEVDASHDMQNKKAKESETDIEQDPGY
  VTPVEHEEELKRFLEREDENCPKQRADNYHQDAGNRDQNTDYLIGLDGEDALLKHLARQSLIVKDALLDR
  TEVPISVEELSCAYELNLFSPRTPPKRQPNGTCEPNPRLNFYPAFAVPEVLATYHIFFKNQKIPVSCRAN
  RTRADALLNLGPGSRLPDIASLEEVPKIFEGLGNNETRAANALQKGENGMDEHHSVLVELEGDNARLAVL
  KRSVEVTHFAYPAVNLPPKVMTAVMDQLLIKRASPLSEDMHDPDACDEGKPVVSDEQLTRWLGTDSPRDL
  EERRKLMMAVVLVTVELECLRRFFTDSETLRKLEENLHYTFRHGFVRQACKISNVELTNLVSYMGILHEN
  RLGQSVLHSTLKGEARRDYIRDCVYLYLCHTWQTGMGVWQQCLEEQNLKELDKLLQKSLKVLWTGFDERT
  VASDLADLIFPERLRVTLRNGLPDFMSQSMLNNFRSFILERSGILPATCCALPSDFVPLTYRECPPPLWS
  HCYLFRLANYLSYHSDVIEDVSGDGLLECHCRCNLCTPHRSLACNPQLMSETQIIGTFELQGPSSQGDGS
  SPGQSLKLTPGLWTSAYLRKFAPEDYHPYEIKFYEDQSQPPKAELSACVITQGAILAQLQAIQKSRQEFL
  LKKGKGVYLDPQTGEELNTRFPQDVPTTRKQEVEGAAAAPRRYGGRLGQSGRGGGGGQSGGQSGGRQFGG
  GKRGGRGGGRSNRRQTVILGCGDKQQRYHLRSESRNPAASQQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 2436 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000588
• RefSeq ORF: 2436
• MW: 91.0 kDa