E3 RID-beta (AC_000019) Virus Tagged ORF Clone

CAT#: VC100100

Myc-DDK-tagged ORF clone of viral ORF for E3 RID-beta [Human adenovirus 35], codon optimized for human cell expression, AP_000598

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3 RID-beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100100 represents NCBI reference of AP_000598 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCCATCCTGCCCATCTTCCTCTTGCTGCTCCTCCCCTACGCCGTATCTACCCCTGCAGCTTACT
  CTACTCCCCCCGAACATCTTAGAAAATGCAAATTCCAACAGCCCTGGAGCTTTCTGGCCTGCTACAGAGA
  AAAGTCCGAAATTCCCCCGAATTTGATCATGATCGCAGGCATCATTAACATCATTTGCTGTACCATTATC
  TCCTTTCTGATCTATCCACTGTTTGACTTCGGTTGGAATGCTCCCAATGCCCATGATCACCCACAAGATC
  CTGAAGAGCATATTCCACCGCAGAACATGCAGCACCCAATTGCTCTCATCGACTACGAAAGCGAGCCTCA
  GCCACCACTCCTGCCCGCTATTTCATACTTCAATCTTACGGGAGGGGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100100 representing AP_000598
  Red=Cloning sites Green=Tags
  
  MQAILPIFLLLLLPYAVSTPAAYSTPPEHLRKCKFQQPWSFLACYREKSEIPPNLIMIAGIINIICCTII
  SFLIYPLFDFGWNAPNAHDHPQDPEEHIPPQNMQHPIALIDYESEPQPPLLPAISYFNLTGGDD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 402 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000598
• RefSeq ORF: 402
• MW: 15.2 kDa