pVII (AC_000019) Virus Tagged ORF Clone

CAT#: VC100083

Myc-DDK-tagged ORF clone of viral ORF for pVII [Human adenovirus 35], codon optimized for human cell expression, AP_000581

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: pVII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100083 represents NCBI reference of AP_000581 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCATACTTATTTCTCCCAGCAATAATACGGGCTGGGGCCTTAGAGCTCCAAGCAAGATGTATGGTG
  GGGCACGGAAAAGGAGCACTCAGCACCCCGTGAGGGTACGAGGACACTTCAGAGCCCCATGGGGAGCCCT
  CAAAGGAAGGACCCGGGTCCGAACGACAGTAGATGACGTCATCGATCAGGTCGTGGCTGACGCTCGGAAC
  TATACCCCAACGGCCCCGACTAGTACAGTGGATGCCGTGATCGACTCAGTCGTGGCAGACGCCCGGAACT
  ACGCCAGGAGAAAGAGCCGCAGGAGAAGGATCGCTAGGAGGCATCGCGCAACAACTGCCATGAGAGCCGC
  TCGAGCACTGCTTAGGAGAGCACGACGAGTAGGCCGAAGGGCAATGCTCAGAGCCGCTCGGAGGGCGGCT
  TCTGGGGCTTCCGCCGGCAGGTCCCGCAGACAAGCTGCTGCTGTGGCAGCAGCTACGATCGCAGATATGG
  CACAGAGCAGGCGGGGAAATGTATACTGGGTCCGGGACGCCGCTACTGGACAGCGCGTTCCCGTGAGAAC
  AAGGCCACCAAGGACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100083 representing AP_000581
  Red=Cloning sites Green=Tags
  
  MSILISPSNNTGWGLRAPSKMYGGARKRSTQHPVRVRGHFRAPWGALKGRTRVRTTVDDVIDQVVADARN
  YTPTAPTSTVDAVIDSVVADARNYARRKSRRRRIARRHRATTAMRAARALLRRARRVGRRAMLRAARRAA
  SGASAGRSRRQAAAVAAATIADMAQSRRGNVYWVRDAATGQRVPVRTRPPRT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 576 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000581
• RefSeq ORF: 576
• MW: 21.3 kDa