E3 CR1-beta1 (AC_000019) Virus Tagged ORF Clone

CAT#: VC100097

Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-beta1 [Human adenovirus 35], codon optimized for human cell expression, AP_000595

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E3 CR1-beta1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100097 represents NCBI reference of AP_000595 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCCTTACAAGTCTTATTTTTGTGAGTATCGTGACCGCCGCACACGGGCAGACAGTCGTTAGCA
  TTCCACTTGGACACAATTACACCCTGATCGGCCCGCCTATTACCTCAGAAGTCATATGGACGAAGTTGGG
  GTCAGTCGATTATTTCGACATCATCTGTAACAAGACTAAGCCGATAATCGTCACCTGCAACATTCAAAAC
  TTGACTCTGATCAACGTCAGTAAGGTGTACAGCGGTTACTATTACGGGTACGACAGGTACTCCTCCCAGT
  ACCGAAATTACCTCGTTAGAGTCACTCAGCTGAAGACAACAAAAATGCCCAACATGGCCAAAATACGATC
  CGACGACAATTCTCTCGAAACCTTTACCTCACCTACAACCCCAGACGAAAAGAACATACCAGACTCAATG
  ATTGCCATCGTGGCTGCTGTGGCCGTGGTAATGGCCCTCATAATTATTTGCATGCTTCTTTATGCATGCC
  GCTACAAGAAATTCCACCCTAAGAAGCAAGACCTGCTGCTCCGACTCAATATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100097 representing AP_000595
  Red=Cloning sites Green=Tags
  
  MASLTSLIFVSIVTAAHGQTVVSIPLGHNYTLIGPPITSEVIWTKLGSVDYFDIICNKTKPIIVTCNIQN
  LTLINVSKVYSGYYYGYDRYSSQYRNYLVRVTQLKTTKMPNMAKIRSDDNSLETFTSPTTPDEKNIPDSM
  IAIVAAVAVVMALIIICMLLYACRYKKFHPKKQDLLLRLNI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 543 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000595
• RefSeq ORF: 543
• MW: 20.4 kDa