22K (AC_000019) Virus Tagged ORF Clone

CAT#: VC100092

Myc-DDK-tagged ORF clone of viral ORF for 22K [Human adenovirus 35], codon optimized for human cell expression, AP_000590

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 22K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100092 represents NCBI reference of AP_000590 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACAAAGGCGCGAGAATAAAAAACTGAAGGTACAGCCTCCTCCCCCAGAAGACATGGAGGAAGACT
  GGGACAGTCAGGCAGAAGAAGCCGAGGAGGACAGCTTGGAGGATTCTCTTGAGGAGGACTCCCTTGAAGA
  AGAGAATGAGGAGGCCGAGGAAGTAGAGGAGGTTACTGCAGACAAGCAGCTGTCCTCTGCTGCAGAAACC
  TCCAACTCCGCAACCATTTCTGCACCATCTCGAGGTACGCGCAGAAGACCATCTTCTCGCTGGGATGAGA
  CGGGAAGGTTTCCTAATCCCACTTCTGCCAGCAAGACTGGCAAAAAAGACCGCCAGGGATACAAGAGCTG
  GCGGGGCCACAAAAACGCAATCATCAGCTGTCTCCACGAGTGCGGCGGGAACATTTCATTTACCCGACGG
  TATTTGCTTTTTCACCACGGTGTCAATTTTCCTCGGAATGTGCTTCACTATTACCGCCATCTCCACAGCC
  CATACTATTCTCAGCAAATCCCAACCGTGTCAACTGATAAAGACAGTGGAGGGGATCTTCAGCAAAAAAC
  CTCAAGTGGGTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100092 representing AP_000590
  Red=Cloning sites Green=Tags
  
  MSQRRENKKLKVQPPPPEDMEEDWDSQAEEAEEDSLEDSLEEDSLEEENEEAEEVEEVTADKQLSSAAET
  SNSATISAPSRGTRRRPSSRWDETGRFPNPTSASKTGKKDRQGYKSWRGHKNAIISCLHECGGNISFTRR
  YLLFHHGVNFPRNVLHYYRHLHSPYYSQQIPTVSTDKDSGGDLQQKTSSGS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 573 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000590
• RefSeq ORF: 573
• MW: 21.7 kDa