III (AC_000019) Virus Tagged ORF Clone

CAT#: VC100082

Myc-DDK-tagged ORF clone of viral ORF for III [Human adenovirus 35], codon optimized for human cell expression, AP_000580

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: III
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100082 represents NCBI reference of AP_000580 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCGGGTGGTGCTGGGAGGAGCTGTGGTTTACCCCGAGGGGCCTCCGCCTAGCTACGAGTCAGTCA
  TGCAGCAGCAACAAGCCACCGCAGTGATGCAATCTCCGCTGGAGGCTCCGTTTGTACCCCCCCGCTACCT
  CGCCCCTACCGAAGGCAGAAATAGCATTCGATACTCCGAACTTGCCCCGCAGTACGACACAACTAGACTC
  TACCTTGTAGACAATAAAAGCGCCGACATCGCGAGCCTGAACTACCAGAATGACCATTCCAACTTCCTGA
  CAACTGTGGTCCAGAACAACGACTTCACACCCACTGAGGCATCTACTCAGACTATCAATTTTGATGAAAG
  GTCTAGGTGGGGTGGCCAGCTGAAGACCATTATGCACACCAACATGCCCAACGTCAATGAGTATATGTTC
  AGCAATAAATTTAAAGCCCGAGTAATGGTCTCAAGAAAGCCCCCTGATGGAGCTGCTGTAGGTGACACTT
  ACGATCACAAACAGGACATCCTGGAATACGAATGGTTCGAGTTCACCTTGCCTGAGGGTAACTTTTCAGT
  GACGATGACTATTGACCTGATGAATAACGCCATTATTGATAATTACCTTAAGGTGGGTCGCCAGAACGGT
  GTGCTGGAGTCCGACATTGGTGTGAAGTTTGACACTCGCAATTTCAAGCTGGGCTGGGATCCCGAGACTA
  AACTGATAATGCCAGGTGTTTATACTTATGAGGCCTTTCACCCAGATATCGTCCTCCTGCCTGGATGTGG
  CGTGGACTTCACCGAAAGCCGACTGAGCAACCTTCTGGGAATAAGGAAAAAACAGCCCTTTCAGGAAGGG
  TTCAAGATACTGTATGAGGATCTGGAAGGCGGAAACATCCCCGCACTGTTGGACGTTGATGCATATGAGA
  ACAGCAAGAAGGAACAAAAAGCCAAAATTGAGGCTGCAACAGCTGCTGCTGAAGCCAAGGCGAACATCGT
  GGCATCAGACTCTACAAGGGTGGCCAACGCTGGCGAGGTGCGAGGAGACAACTTTGCCCCCACTCCCGTA
  CCAACCGCGGAGAGTCTCCTCGCAGATGTGAGTGAGGGGACAGATGTGAAGCTTACCATCCAGCCCGTGG
  AGAAAGACTCAAAAAATAGAAGCTACAACGTCCTGGAGGATAAGATTAATACCGCCTACAGATCATGGTA
  CCTTAGCTATAATTACGGAGATCCAGAGAAGGGCGTCAGGTCCTGGACACTCCTGACAACCTCCGATGTG
  ACATGCGGAGCGGAACAGGTATACTGGTCATTGCCTGACATGATGAAAGACCCAGTTACATTTAGGTCAA
  CAAGACAAGTGTCCAACTATCCAGTGGTCGGCGCCGAACTCATGCCAGTGTTTAGTAAGAGCTTCTATAA
  CGAACAGGCTGTGTACTCTCAGCAGCTCAGACAGTCAACCTCTCTGACCCACGTGTTCAATCGCTTTCCA
  GAAAACCAGATCCTCATCAGGCCTCCAGCTCCAACAATTACAACAGTGAGTGAGAATGTACCCGCCTTGA
  CGGATCATGGAACACTCCCACTCCGGTCATCCATACGGGGAGTCCAGAGGGTTACGGTGACAGATGCCAG
  ACGACGCACCTGCCCATATGTGTATAAAGCCCTTGGTATCGTTGCTCCTAGAGTCCTGTCAAGTCGCACG
  TTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100082 representing AP_000580
  Red=Cloning sites Green=Tags
  
  MRRVVLGGAVVYPEGPPPSYESVMQQQQATAVMQSPLEAPFVPPRYLAPTEGRNSIRYSELAPQYDTTRL
  YLVDNKSADIASLNYQNDHSNFLTTVVQNNDFTPTEASTQTINFDERSRWGGQLKTIMHTNMPNVNEYMF
  SNKFKARVMVSRKPPDGAAVGDTYDHKQDILEYEWFEFTLPEGNFSVTMTIDLMNNAIIDNYLKVGRQNG
  VLESDIGVKFDTRNFKLGWDPETKLIMPGVYTYEAFHPDIVLLPGCGVDFTESRLSNLLGIRKKQPFQEG
  FKILYEDLEGGNIPALLDVDAYENSKKEQKAKIEAATAAAEAKANIVASDSTRVANAGEVRGDNFAPTPV
  PTAESLLADVSEGTDVKLTIQPVEKDSKNRSYNVLEDKINTAYRSWYLSYNYGDPEKGVRSWTLLTTSDV
  TCGAEQVYWSLPDMMKDPVTFRSTRQVSNYPVVGAELMPVFSKSFYNEQAVYSQQLRQSTSLTHVFNRFP
  ENQILIRPPAPTITTVSENVPALTDHGTLPLRSSIRGVQRVTVTDARRRTCPYVYKALGIVAPRVLSSRT
  F
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 1683 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000580
• RefSeq ORF: 1683
• MW: 62.9 kDa