52K (AC_000019) Virus Tagged ORF Clone

CAT#: VC100080

Myc-DDK-tagged ORF clone of viral ORF for 52K [Human adenovirus 35], codon optimized for human cell expression, AP_000578

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 52K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100080 represents NCBI reference of AP_000578 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCCTGTGCTGAGACAGATGAGACCCCAGCAACAGCCACCCTCACAGCAGCAACAACAACAGCCCC
  AAAAGGCAGTGCCCGCAACCACGGCGACCGCCGCTGTGTCTGGCGCAGGCCAGCCAGCATATGATTTGGA
  CCTCGAGGAAGGAGAGGGACTGGCCAGACTCGGGGCTCCCTCACCAGAAAGACACCCCCGAGTGCAACTG
  AAAAAGGATAGTCGCGAAGCATATGTACCTCAGCAGAATCTGTTTAGGGACCGATCTGGAGAGGAACCTG
  AGGAGATGCGGGCTAGTCGGTTCAACGCCGGCCGCGAATTGAGGCACGGCCTCGACAGGCGGAGGGTGCT
  GCGAGATGAGGACTTCGAGGTGGACGAAGTCACTGGGATCAGTCCCGCACGCGCTCATGTTGCTGCAGCT
  AATCTGGTTAGTGCATATGAGCAGACCGTCAAGGAGGAGCGAAATTTCCAGAAGAGTTTTAACAACCATG
  TGCGCACTCTGATAGCAAGGGAGGAGGTTACGCTGGGCCTGATGCACCTTTGGGACCTGATGGAAGCCAT
  CATCCAAAACCCCACCTCTAAGCCACTTACAGCCCAGTTGTTTCTCGTCGTGCAGCACTCCCGGGATAAC
  GAGGCTTTCCGCGAGGCGCTGCTGAACATCACGGAGCCCGAAGGAAGGTGGCTGTACGACCTGATCAATA
  TTCTGCAAAGTATAATCGTGCAGGAACGGAGTTTGGGTCTGGCCGAGAAGGTAGCCGCAATCAATTATTC
  CGTCCTCTCACTGGGAAAATACTACGCCCGCAAAATTTACAAGACCCCTTATGTCCCGATCGACAAAGAG
  GTCAAGATTGACGGATTCTACATGCGCATGACACTCAAAGTGCTCACACTCTCAGACGACCTGGGGGTCT
  ATCGGAATGACCGGATGCATCGCGCGGTGAGTGCCAGCAGGAGACGCGAGCTCTCCGACCGCGAGCTTAT
  GCATTCCCTGCAGAGAGCACTGACAGGAGCTGGGACGGAGGGTGAGAACTACTTCGATATGGGCGCCGAT
  CTCCAGTGGCAACCAAGCAGACGGGCCTTGAGCGCAGCGACCGCTGGCTGTGAGCTCCCTTACATCGAGG
  AAGCCGACGAAGGTGAAGAGGAGGAGGGAGAATATCTTGAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100080 representing AP_000578
  Red=Cloning sites Green=Tags
  
  MHPVLRQMRPQQQPPSQQQQQQPQKAVPATTATAAVSGAGQPAYDLDLEEGEGLARLGAPSPERHPRVQL
  KKDSREAYVPQQNLFRDRSGEEPEEMRASRFNAGRELRHGLDRRRVLRDEDFEVDEVTGISPARAHVAAA
  NLVSAYEQTVKEERNFQKSFNNHVRTLIAREEVTLGLMHLWDLMEAIIQNPTSKPLTAQLFLVVQHSRDN
  EAFREALLNITEPEGRWLYDLINILQSIIVQERSLGLAEKVAAINYSVLSLGKYYARKIYKTPYVPIDKE
  VKIDGFYMRMTLKVLTLSDDLGVYRNDRMHRAVSASRRRELSDRELMHSLQRALTGAGTEGENYFDMGAD
  LQWQPSRRALSAATAGCELPYIEEADEGEEEEGEYLED
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 1164 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000578
• RefSeq ORF: 1164
• MW: 44.1 kDa