33K (AC_000019) Virus Tagged ORF Clone

CAT#: VC100091

Myc-DDK-tagged ORF clone of viral ORF for 33K [Human adenovirus 35], codon optimized for human cell expression, AP_000589

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 33K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100091 represents NCBI reference of AP_000589 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCAACGGAGGGAAAATAAAAAGTTGAAAGTTCAGCCGCCCCCTCCAGAAGATATGGAAGAAGATT
  GGGACTCCCAAGCTGAAGAGGCAGAAGAGGATAGTCTTGAAGATAGTCTGGAAGAGGATTCTCTTGAGGA
  AGAGAATGAAGAAGCCGAAGAGGTAGAAGAGGTAACCGCTGACAAACAGCTCAGTTCAGCTGCCGAGACC
  TCCAACAGTGCGACTATCTCCGCCCCAAGTCGAGGAACGAGACGCAGGCCCAGTTCTCGCTGGGATGAAA
  CAGGCCGATTTCCAAACCCTACCTCAGCTAGCAAGACCGCTCCCACAATCGCTAGCAAGTCTCGCCAGTC
  AAGACAGATCAAAACCGCGGCTGCAACCAGCAACAGGAAACCGGCTGCTGCCGTAAGGAAGTATACCACT
  TCTGCCGCAACCGGGGGGCTCAAAATTACTGCCAATGAGCCCGCCCAGACTCGGGAGCTGCGGAATAGGA
  TCTTCCCGACTCTTTACGCCATCTTTCAGCAGAGTCGAGGACAGGAGCAGGAACTGAAAATCAAAAATAG
  GAGCCTGAGGAGCCTGACAAGGTCTTGCCTCTATCACAAATCTGAGGATCAGCTGCAACGCACTCTGGAA
  GACGCCGAGGCACTTTTTAACAAGTATTGTGCGCTTACCCTCAAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100091 representing AP_000589
  Red=Cloning sites Green=Tags
  
  MSQRRENKKLKVQPPPPEDMEEDWDSQAEEAEEDSLEDSLEEDSLEEENEEAEEVEEVTADKQLSSAAET
  SNSATISAPSRGTRRRPSSRWDETGRFPNPTSASKTAPTIASKSRQSRQIKTAAATSNRKPAAAVRKYTT
  SAATGGLKITANEPAQTRELRNRIFPTLYAIFQQSRGQEQELKIKNRSLRSLTRSCLYHKSEDQLQRTLE
  DAEALFNKYCALTLKE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 678 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000589
• RefSeq ORF: 678
• MW: 25.4 kDa