E1A (AC_000019) Virus Tagged ORF Clone

CAT#: VC100073

Myc-DDK-tagged ORF clone of viral ORF for E1A [Human adenovirus 35], codon optimized for human cell expression, AP_000571

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100073 represents NCBI reference of AP_000571 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGATCTCCGGTTTCTGCCTCAAGAGATTATCTCAGCTGAAACAGGGAATGAGATCCTCGAGCTTG
  TGGTGCATGCCCTGATGGGCGACGATCCAGAACCTCCCGTCCAGTTGTTTGAGCCCCCTACCCTTCAGGA
  GCTTTATGATCTGGAGGTGGAAGGCAGTGAGGATTCCAACGAGGAGGCCGTGAACGGCTTTTTTACAGAT
  AGCATGTTGCTGGCTGCCAATGAGGGACTGGAACTCGACCCTCCTCTGGACACTTTCAATACCCCGGGAG
  TGATCGTGGAGTCCGGGACTGGCGTGCGCAAGCTCCCGGATCTGTCCAGCGTGGACTGCGATTTGCATTG
  CTATGAAGATGGCTTCCCACCTAGCGACGAGGAGGACCACGAAAAAGAGCAGTCTATGCAGACCGCCGCC
  GGCGAGGGTGTCAAAGCTGCTAACGTGGGCTTTCAGTTGGATTGTCCAGAACTGCCAGGGCACGGGTGCA
  AATCCTGCGAATTCCACCGGAAAAATACCGGGGTGAAGGAGTTGCTGTGCTCCCTCTGTTACATGAGGAC
  CCACTGTCACTTTATCTACTCACCTGTTTCCGACGCCGACGAGTCACCTAGCCCGGACAGCACCACCTCT
  CCTCCCGACATCCAGGCACCTGTACCAGTGGACGTGAGGAAACCAATTCCTGTCAAGCTGAAGCCAGGTA
  AGCGGCCTGCAGTCGAAAAACTGGAAGACCTGCTCCAGGGGGGGGATGGCCCTTTGGATTTGAGCACGCG
  GAAACGCCCCCGCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100073 representing AP_000571
  Red=Cloning sites Green=Tags
  
  MRDLRFLPQEIISAETGNEILELVVHALMGDDPEPPVQLFEPPTLQELYDLEVEGSEDSNEEAVNGFFTD
  SMLLAANEGLELDPPLDTFNTPGVIVESGTGVRKLPDLSSVDCDLHCYEDGFPPSDEEDHEKEQSMQTAA
  GEGVKAANVGFQLDCPELPGHGCKSCEFHRKNTGVKELLCSLCYMRTHCHFIYSPVSDADESPSPDSTTS
  PPDIQAPVPVDVRKPIPVKLKPGKRPAVEKLEDLLQGGDGPLDLSTRKRPRQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 786 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000571
• RefSeq ORF: 786
• MW: 28.7 kDa