E1B 55K (AC_000019) Virus Tagged ORF Clone

CAT#: VC100075

Myc-DDK-tagged ORF clone of viral ORF for E1B 55K [Human adenovirus 35], codon optimized for human cell expression, AP_000573

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1B 55K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100075 represents NCBI reference of AP_000573 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCAGCCGACTCCTTCCAGCAGGGAATCCGCTTCGGTTTCCACTCCCACAGCATTGTGGAGAATA
  TGGAAGGCAGTCAAGATGAAGACAATCTGCGCCTGCTCGCTTCAGCCGCATTTGGATGCAGCGGGAACCC
  AGAAGCATCCACGGGCCATGCCAGTGGAAGTGGTGGTGGAACCGCCAGGGGGCAGCCTGAAAGTCGCCCC
  GGTCCATCTTCCGGTGGGGGCGGGGTGGCAGACCTTTCACCCGAACTTCAAAGAGTGCTCACCGGCAGCA
  CAAGTACCGGTCGGGATCGGGGTGTTAAACGCGAGCGCGCTAGCTCTGGTACGGATGCGCGGAGCGAGCT
  CGCTTTGTCTCTTATGAGCCGGAGAAGGCCCGAAACCATCTGGTGGCACGAAGTCCAAAAGGAGGGCCGG
  GATGAGGTATCTGTGCTGCAGGAGAAATATAGCCTCGAGCAGGTTAAAACGTGCTGGCTGGAGCCAGAAG
  ACGATTGGGCCGTGGCGATTAAAAATTATGCCAAGATAGCCCTGCGGCCCGATAAGCAGTACAAAATAAG
  CAGGAGGATTAACATCCGGAATGCCTGTTATATCTCTGGGAACGGTGCAGAGGTCGTCATCGACACACAG
  GATAAAACTGTGATCAGATGTTGCATGATGGATATGTGGCCAGGCGTCGTGGGGATGGAGGCCGTGACAT
  TCGTCAACGTCAAGTTTCGGGGTGACGGCTACAATGGCATCGTGTTCATGGCAAATACCAAACTTATCCT
  CCACGGATGCAGTTTTTTCGGCTTCAACAATACCTGCGTCGACGCCTGGGGTCAAGTGTCCGTGAGGGGT
  TGTTCCTTTTATGCTTGTTGGATTGCCACTGCCGGACGCACTAAGTCACAGCTGAGCTTGAAAAAATGTA
  TATTCCAACGGTGCAATCTCGGTATTCTGAATGAAGGAGAAGCCAGAGTGAGGCACTGTGCCTCAACCGA
  TACCGGATGTTTTATTTTGATCAAGGGCAACGCTTCCGTGAAGCACAACATGATCTGTGGTGCCAGTGAT
  GAGAGACCCTATCAAATGCTTACCTGCGCGGGTGGTCACTGCAACATGCTTGCCACCGTGCATATCGTAT
  CACACCAGCGCAAGAAGTGGCCAGTATTCGACCACAATGTTCTCACGAAATGTACGATGCACGCTGGAGG
  GAGGCGAGGGATGTTTATGCCTTACCAGTGCAATATGAACCACGTGAAAGTCCTCCTTGAGCCAGATGCC
  TTCAGCAGAATGTCTCTGACAGGAATATTCGACATGAACACTCAGATCTGGAAAATCCTGAGGTACGACG
  ATACCAGATCAAGAGTGCGGGCCTGTGAATGTGGTGGGAAGCACGCGAGGTTTCAGCCCGTCTGCGTAGA
  TGTAACCGAAGATCTGAGACCCGATCACCTGGTGATCGCGAGGACAGGGGCTGAATTCGGCAGCAGCGGT
  GAAGAGACTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100075 representing AP_000573
  Red=Cloning sites Green=Tags
  
  MDPADSFQQGIRFGFHSHSIVENMEGSQDEDNLRLLASAAFGCSGNPEASTGHASGSGGGTARGQPESRP
  GPSSGGGGVADLSPELQRVLTGSTSTGRDRGVKRERASSGTDARSELALSLMSRRRPETIWWHEVQKEGR
  DEVSVLQEKYSLEQVKTCWLEPEDDWAVAIKNYAKIALRPDKQYKISRRINIRNACYISGNGAEVVIDTQ
  DKTVIRCCMMDMWPGVVGMEAVTFVNVKFRGDGYNGIVFMANTKLILHGCSFFGFNNTCVDAWGQVSVRG
  CSFYACWIATAGRTKSQLSLKKCIFQRCNLGILNEGEARVRHCASTDTGCFILIKGNASVKHNMICGASD
  ERPYQMLTCAGGHCNMLATVHIVSHQRKKWPVFDHNVLTKCTMHAGGRRGMFMPYQCNMNHVKVLLEPDA
  FSRMSLTGIFDMNTQIWKILRYDDTRSRVRACECGGKHARFQPVCVDVTEDLRPDHLVIARTGAEFGSSG
  EETD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000019
• ORF Size: 1482 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: AC_000019.1, AP_000573
• RefSeq ORF: 1482
• MW: 54.6 kDa